Primary Information
BoMiProt ID	Bomi4731
Protein Name	Clathrin heavy chain 1
Organism	Bos taurus
Uniprot ID	P49951
Milk Fraction	Whey
Ref Sequence ID	NP_776448.1
Aminoacid Length	1675
Molecular Weight	191589
FASTA Sequence	Download
Gene Name	CLTC
Gene ID	281080
Protein Existence Status	reviewed
Secondary Information
Protein Function	Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep
Biochemical Properties	The N-terminal domain ~NTD! of clathrin heavy chain 1 ~CLTC! is important for clathrin to bind to the spindle, and studies indicate that NTD and the trimerization domain of CTLC are essential for its mitotic function
PTMs	Phosphorylation at Ser/Thr
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|P49951|CLH1_BOVIN Clathrin heavy chain 1 OS=Bos taurus OX=9913 GN=CLTC PE=1 SV=1 MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSN PIRRPIS*67ADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMT*105DDVTFWKWISLNTVA LVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGA MQLY*184SVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGN QPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISG ETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGA EELFARKFNALFAQGNYSEAAKVAANAPKGILRT*394PDTIRRFQSVPAQPGQTSPLLQYFGI LLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYL RANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDE EPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAIL GNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLY*634DIKRAVVHTHLLNPEWLVNYFGSLSV EDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVN FSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDR FDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQF STDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPY*899Y DSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVL LESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSE HRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTS AVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEV VQAANTSGNWEELVKYLQMARKKARES*1167YVETELIFALAKTNRLAELEEFINGPNNAHIQQ VGDRCY*1206DEKMYDAAKLLYNNVSNFGRLAS*1229TLVHLGEYQAAVDGARKANSTRTWKEVCFAC VDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTEL AILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMN HPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFS KVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDY*1477QALRTSIDAY*1487DNFDNIS*1494LAQRLE KHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQE EKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLR KEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPAYGQPQPGFGYSM
SCOP	Class : All alpha proteins Fold : alpha-alpha superhelix Superfamily : ARM repeat-like Family : Clathrin heavy chain proximal leg segment Domain Name : 1B89 A:1182-1517 Class : All alpha proteins Fold : alpha-alpha superhelix Superfamily : ARM repeat-like Family : Clathrin heavy-chain linker domain Domain Name : 1UTC A:331-355 Class : All beta proteins Fold : 7-bladed beta-propeller Superfamily : Clathrin heavy-chain terminal domain-like Family : Clathrin heavy-chain terminal domain Domain Name : 1UTC A:4-330
CATH	Matched CATH superfamily 1.25.40.10 1.25.40.730 2.130.10.110
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
PDB ID	1B89,1UTC,1XI4,1XI5,3GC3,3GD1,3IYV,3LVG,3LVH,3QIL,5M5R,5M5S,5M5T,5M5U,5M5V,5M61,6WCJ,
Additional Comments	CLTC plays important roles in both spindle assembly and chromosome congression by interacting with cytoskeleton-associated protein 5 (CKAP5)
Bibliography	1.Han Z, Hao X, Zhou CJ, Wang J, Wen X, Wang XY, Zhang DJ, Liang CG. Clathrin Heavy Chain 1 Plays Essential Roles During Oocyte Meiotic Spindle Formation and Early Embryonic Development in Sheep. Front Cell Dev Biol. 2021 Feb 25;9:609311. doi: 10.3389/fcell.2021.609311. PMID: 33718352; PMCID: PMC7946971. 2.Han, Z., Liang, C. G., Cheng, Y., Duan, X., Zhong, Z., Potireddy, S., et al. (2010). Oocyte spindle proteomics analysis leading to rescue of chromosome congression defects in cloned embryos. J. Proteome Res. 9, 6025–6032. doi: 10.1021/pr100827j. 3.Royle, S.J. & Lagnado, L. ~2006!. Trimerisation is important for the function of clathrin at the mitotic spindle. J Cell Sci 119~Pt 19!, 4071–4078.