|
|
|
Primary Information | |
|---|---|
| BoMiProt ID | Bomi4707 |
| Protein Name | Chymosin/Preprorennin |
| Organism | Bos taurus |
| Uniprot ID | P00794 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_851337.1 |
| Aminoacid Length | 381 |
| Molecular Weight | 42180 |
| FASTA Sequence | Download |
| Gene Name | CYM/CPC |
| Gene ID | 529879 |
| Protein Existence Status | reviewed |
Secondary Information | |
| Presence in other biological fluids/tissue/cells | in the abomasum of calves and the stomach of human infants which clots milk by precipitation of the casein |
| Protein Function | Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzymehas endopeptidase activity which hydrolyzes casein to paracasein. |
| Biochemical Properties | Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.The substrate affinity and catalytic activity of bovine chymosin increase at lower pH (6.00 and 5.50).Chymosin proteolytically cuts and inactivates kappa casein, converting it into para-kappa-casein and a smaller protein called macropeptide. Para-kappa-casein does not have the ability to stabilize the micellar structure and the calcium-insoluble caseins precipitate, forming a curd. |
| Significance in milk | is secreted in the neonatal stomach of ruminants.Function is to curdle or coagulate milk.chymosin is also a very important industrial enzyme because it is widely used in cheesemaking. |
| PTMs | Disulfide bond formation,Proteolytic cleavage |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | na |
| SCOP | Class : All beta proteins Fold : Acid proteases Superfamily : Acid proteases Family : Pepsin-like Domain Name : 1CMS A:1-323 |
| CATH | Matched CATH superfamily 2.40.70.10 |
| Predicted Disorder Regions | NA |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| PDB ID | 1CMS,1CZI,3CMS,4AA8,4AUC,4CMS, |
| Bibliography | Jensen JL, Jacobsen J, Moss ML, Rasmussen F, Qvist KB, Larsen S, van den Brink JM. The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay. J Dairy Sci. 2015 May;98(5):2853-60. doi: 10.3168/jds.2014-8672. Epub 2015 Feb 26. PMID: 25726113. |