Search by BoMiProt ID - Bomi4707

Primary Information

BoMiProt ID Bomi4707
Protein Name Chymosin/Preprorennin
Organism Bos taurus
Uniprot IDP00794
Milk FractionWhey
Ref Sequence ID NP_851337.1
Aminoacid Length 381
Molecular Weight 42180
FASTA Sequence Download
Gene Name CYM/CPC
Gene ID 529879
Protein Existence Status reviewed

Secondary Information

Presence in other biological fluids/tissue/cells in the abomasum of calves and the stomach of human infants which clots milk by precipitation of the casein
Protein Function Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzymehas endopeptidase activity which hydrolyzes casein to paracasein.
Biochemical Properties Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.The substrate affinity and catalytic activity of bovine chymosin increase at lower pH (6.00 and 5.50).Chymosin proteolytically cuts and inactivates kappa casein, converting it into para-kappa-casein and a smaller protein called macropeptide. Para-kappa-casein does not have the ability to stabilize the micellar structure and the calcium-insoluble caseins precipitate, forming a curd.
Significance in milk is secreted in the neonatal stomach of ruminants.Function is to curdle or coagulate milk.chymosin is also a very important industrial enzyme because it is widely used in cheesemaking.
PTMs Disulfide bond formation,Proteolytic cleavage
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
SCOP Class : All beta proteins
Fold : Acid proteases
Superfamily : Acid proteases
Family : Pepsin-like
Domain Name : 1CMS A:1-323

CATH Matched CATH superfamily
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography Jensen JL, Jacobsen J, Moss ML, Rasmussen F, Qvist KB, Larsen S, van den Brink JM. The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay. J Dairy Sci. 2015 May;98(5):2853-60. doi: 10.3168/jds.2014-8672. Epub 2015 Feb 26. PMID: 25726113.