Primary Information |
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| BoMiProt ID | Bomi4674 |
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| Protein Name | Chromatin assembly factor 1 subunit A/Chromatin assembly factor I p150 subunit/CAF-I 150 kDa subunit/CAF-I p150 |
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| Organism | Bos taurus |
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| Uniprot ID | A6QLA6 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001095312.1 |
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| Aminoacid Length | 964 |
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| Molecular Weight | 107258 |
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| FASTA Sequence |
Download |
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| Gene Name | CHAF1A/CAF/CAF1P150 |
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| Gene ID | 504535 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | CHAF1A by potentially mediating cancer cell proliferation plays an important role in promoting the development of HCC(hepatocellular carcinoma) and may serve as a potential therapeutic target in HCC(hepatocellular carcinoma). |
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| Biochemical Properties | Chromatin assembly factor-1 (CAF-1), an evolutionarily conserved histone H3/H4 chaperone with subunits shown previously to interact with CenH3 in flies and human cells, regulates Cse4 deposition in budding yeast. yCAF-1 interacts with Cse4 and can assemble Cse4 nucleosomes in vitro. |
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| PTMs | Isopeptide bond formation, Phosphorylation at Ser/Thr, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A6QLA6|CAF1A_BOVIN Chromatin assembly factor 1 subunit A OS=Bos taurus OX=9913 GN=CHAF1A PE=2 SV=1
MLEEPECGAPGARGEAAAMDCKDRPAFPVKKLIQARLPFKRLNLVPKEKIDDGLDDTGGS
RAGPVQTQLHNLETSLDHLENCHMGSDIDFRPKLVNGKGPLDNFLRSQVETSIGQAVVII
DLTEDS*126SNPPDNMVGHNKLNS*141AAS*144SAQKNINGVPDKAGDDRGLPKARQKDELASPEEALS
EVPCKTEAGGADSGGADRRGLTQRGSPQNCPKLTGDLSMWSEKDRDGWSEAGGILFKGKM
PVVVLQDILALRPPARSPPATPPSQAVPSESETPESSPEEDLALSHSSLSSSSPTSSPEG
QSVPTKLHTGPS*312PFPASTPVCRITKKLVRGSAEKNKMKLQRDKERLRRQLKLRAEKEEKE
KLREEAKRAKEEARKKREEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAK
LEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMV
LAPRCRTAFDQDLCDQLDQLLQQQSSEFSFLQDLKSRRPLRSGPTVVSNRNTDLSNSDVV
IVESSKVDGVPERRKFGRMKLLQFSENHRPAYWGTWNKKTTVIRPRDPWAQDRDLLDYEV
DSDEEWEEEEPGESLSHSEGDDDDDVGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHK
VRQKLKAKEWDEFLAKGKRFRILQPVKIGCIWAADKDGGADLKVLQQFTACLLETVPPEE
EQT*723PKASKREKRDQQILAQLLPLLHGNVNGSKVIIREFQECCRRGLLSRDAGS*773PEDSAAS
PPS*783PGPARPQTPTASEDVAVPSKARLKRIIS*811ENSVYEKRPDFRMCWYVHPQVLKSFAQEH
LPVPCQWSYVTAVPSATREDSGSVPAPGPGQGMPVS*876LKRKS*881AGSMCITQFMKKRRHDGQV
GTGDLDDFQADTEEEDDDEGDCVIMDISDVGDIQAPCGTTSGAGGSVGMDTSESFVSPS*959S
LRLS
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| Predicted Disorder Regions | 22-26, 157-186, 261-304, 598-646, 771-792, 857-961 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Deletion of CAF-1 p150 subunit impairs the silencing of many genes including Oct4, Sox2 and Nanog as well as the establishment of H3K27me3 at these gene promoters during ESC differentiation. |
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| Bibliography | 1.Hewawasam GS, Dhatchinamoorthy K, Mattingly M, Seidel C, Gerton JL. Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast. Nucleic Acids Res. 2018 May 18;46(9):4440-4455. doi: 10.1093/nar/gky169. Erratum in: Nucleic Acids Res. 2018 May 18;46(9):4831. PMID: 29522205; PMCID: PMC5961020. 2.Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, Zhang Z. Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit. Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 10.1093/nar/gkz858. PMID: 31586391; PMCID: PMC6868363. 3.Xu M, Jia Y, Liu Z, Ding L, Tian R, Gu H, Wang Y, Zhang H, Tu K, Liu Q. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma. Onco Targets Ther. 2016 Jul 1;9:4023-35. doi: 10.2147/OTT.S107050. PMID: 27445493; PMCID: PMC4936808. |
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