Primary Information
BoMiProt ID	Bomi4433
Protein Name	Calpain-3/Calcium-activated neutral proteinase 3/CANP 3/Calpain L3/Calpain p94/Muscle-specific calcium-activated neutral protease 3/New calpain 1/nCL-1
Organism	Bos taurus
Uniprot ID	P51186
Milk Fraction	Whey
Ref Sequence ID	NP_776685.1
Aminoacid Length	822
Molecular Weight	94603
FASTA Sequence	Download
Gene Name	CAPN3/NCL1
Gene ID	281663
Protein Existence Status	reviewed
Secondary Information
Protein Function	Calpain 3 is important for muscle regeneration
Biochemical Properties	The model shows that the proteolytic domain can be subdivided into two globular subdomains (domain IIa and IIb), forming a catalytic cleft at their interface. As in ubiquitous calpains, domain III of calpain 3 fits a C2 motif.In this model, IS1 andIS2 have been structured as loops protruding out of the globular core structure. However, Diaz and colleagues have shown that, instead of protruding, IS1 is composed of an a-helix flanked by loops that close the catalytic cleft, blocking its access to substrates and inhibitors.
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	na
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	Loss-of-function mutations in the calpain 3 gene have been associated with limb-girdle muscular dystrophy type 2A (LGMD2A), a common form of muscular dystrophy .
Bibliography	1.Hauerslev S, Sveen ML, Duno M, Angelini C, Vissing J, Krag TO. Calpain 3 is important for muscle regeneration: evidence from patients with limb girdle muscular dystrophies. BMC Musculoskelet Disord. 2012 Mar 23;13:43. doi: 10.1186/1471-2474-13-43. PMID: 22443334; PMCID: PMC3338386. 2.Duguez S, Bartoli M, Richard I. Calpain 3: a key regulator of the sarcomere? FEBS J. 2006 Aug;273(15):3427-36. doi: 10.1111/j.1742-4658.2006.05351.x. PMID: 16884488.