Primary Information |
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| BoMiProt ID | Bomi4313 |
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| Protein Name | Breast cancer type 1 susceptibility protein homolog/RING-type E3 ubiquitin transferase BRCA1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q864U1 |
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| Milk Fraction | Whey,MFGM |
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| Ref Sequence ID | NP_848668.1 |
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| Aminoacid Length | 1849 |
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| Molecular Weight | 206279 |
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| FASTA Sequence |
Download |
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| Gene Name | BRCA1 |
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| Gene ID | 353120 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | tumor suppressor genes,contribute to DNA repair and transcriptional regulation in response to DNA damage. |
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| Significance in milk | Its Presence in milk is important for mastitis resistance in cattle. |
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| PTMs | Phosphorylation,Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q864U1|BRCA1_BOVIN Breast cancer type 1 susceptibility protein homolog OS=Bos taurus OX=9913 GN=BRCA1 PE=1 SV=1
MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQ
CPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKEDNS*114PEHLKE
EVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVELSNLGIVRSLRTKQQTQSQNKSVYI
ELGSDSSEDTVNKASYFSVGDHELLEITPQGAKAKTNLNPAEKAACEFSEKDITNTEHHQ
LSIKDLITTQKHATETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVE
KAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTEKKIVLNTDPLYRRKELRKQKPAC
PDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTS*395DDS*398CDGGSESNNEVAGAVEIPNKVD
GYS*423GSSEKINLMAS*434DPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFSHIAEDLI
LGAFTVEPQITQEQPLTNKLKCKRRGTSGLQPEDFIKKVDLTIVPKTPEKMTEGTDQTEQ
KCHGMNITSDGHENKTKRDYVQKEQNANPAESLEKESVFRTEAEPISISISNMELELNIH
RSKAPKNRLKRKSSTRKIPELELVVSRNPSLPNHTELPIDSSSSNEEMKKKHSSQMPVRQ
SQKLQLIGDKELTAGAKNNKTYEQINKRLAS*691DAFPELKLTNTPGYFTNCSS*711KPEEFVHPS*720
LQREENLGTIQVSNSTKDPKDLILREGKALQIERSVESTNISLVPDTDYSTQDSISLLEA
KTPEKAKTAPNPCVSLCTATKNLKELIHRDFKDTKNNTEGFQDLLGHDINYVIQETSREM
EDSELDTQYLQNTFKASKRQTFALFSNPGNPQKECATVFAHSGSLRDQSPRDPLKCRQKE
DSQGKSESKSQHVQAICTTVHFPVADQQDRTPGDDAKCSAKEVTRVCQSSQLRGHKTELV
FANKQGVSEKPNLIPSLSPIKS*982SVKTICKKSPSEKFEEPVTS*1002PEKTLGSESIIQSAVSTI
SQNNIQESTFKEVSSNSVNEVGSSTNEVGSSVNEVGSSGENIQAEPGRNREPKLRALLGL
GLTQPEVYKQSLPVSNCHHPEIKRQGENEDMPQAVKADFSPCLISDNLEQPTGSRHAS*1138QV
CSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGS*1184PGPFTHTHLAQGHQRG
AGKLESEETVS*1211S*1212EDEELPCFQQLLFGKVTSTLSPSTGCNTVATEGLSKETEGNLESLKSG
LNDCSGQVTSAKVS*1274QEHHLNEEARCSGSLFSSQCSAMEDLTTNTNTQDPFLMFERPSKQV
YQS*1323ESEEVLS*1330DKELVS*1336DDEERETGLEEDSCQEEQSVDSDLGEAVSDHVSETSLSEDGVGL
SS*1382QSDILTT*1389QQRDTMQDNLLKLQQEMAELEAVLERHGS*1418QPSHSSASLTADSRGPEHLLNL
EQDTSERAILTS*1452EKSRDYSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSASKFQLSY
NRWYMHSSRSLQDRNCPS*1518QKEPINVADMEEQQLAKREAQDLMGSFLPRQDQEGTPYLKSG
ISLFSHEPESDPSEDRAAEPAHVHSMPPSASALKLSQFRVEESTKNPAAAHIANTTRCNL
REESMSKEKPEVISSTERSKKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHV
IMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKEGKMLDEHDFEVRGDVVNGRNHQ
GPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPDQGTHPV
VVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVP
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| Predicted Disorder Regions | 2 predicted disordered segments; (182-940), (987-1627) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | BRCA1 is hyperphosphorylated in response to DNA damage and relocated to sites of replication forks marked by proliferating cell nuclear antigen (PCNA).In response to ionizing radiation, BRCA1 is bound and phosphorylated by an ataxia-telangiectasia mutated (ATM) kinase.The major target for ATM phosphorylation after ionizing radiation is Ser1387 of BRCA1. In response to UV irradiation, Ser1457 is primarily phosphorylated, mainly by ATM-related kinase (ATR).The G2/M control kinase, CHK2, has been shown to phosphorylate BRCA1 at Ser988 on exposure to ionizing radiation. Ser1423 and Ser1524 are also phosphorylated in response to DNA damage.Lys63, Lys48 and Lys6 polyubiquitin chains can be conjugated to BRCA1.. “Substrate-specific” monoubiquitination by the E2s UbcH6, Ube2e2, UbcM2, Ube2w and UbcH5 result in the conjugation of a single Ub residue to BRCA1.“Ubiquitin-specific” E2s, Ubc13, Ube2k and UbcH5 recognize monoubiquitinated BRCA1 and stimulate the conjugation of Lys6, Lys48, and Lys63 polyubiquitin chains to BRCA1. |
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| Bibliography | 1.Yoshida K, Miki Y. Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage. Cancer Sci. 2004 Nov;95(11):866-71. doi: 10.1111/j.1349-7006.2004.tb02195.x. PMID: 15546503. 2.Yuan Z, Chu G, Dan Y, Li J, Zhang L, Gao X, Gao H, Li J, Xu S, Liu Z. BRCA1: a new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score. Mol Biol Rep. 2012 Jun;39(6):6625-31. doi: 10.1007/s11033-012-1467-5. Epub 2012 Feb 12. PMID: 22327776. |
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