Search by BoMiProt ID - Bomi4202

Primary Information

BoMiProt ID Bomi4202
Protein Name Autophagy-related protein 101
Organism Bos taurus
Uniprot IDQ2HJE0
Milk Fractionwhey
Ref Sequence ID NP_001039599.1
Aminoacid Length 218
Molecular Weight 25017
FASTA Sequence Download
Gene Name ATG101
Gene ID 513049
Protein Existence Status Reviewed

Secondary Information

Protein Function Does Atg13 phosphorylation which helps in phagophore formation.ATG101 plays a critical role in linking ULK1 and PtdIns3K complexes for activation of autophagy pathways.Autophagy-related protein 101 is an autophagy factor which is essential for autophagosome formation. It also stabilizes ATG13 by protecting it from proteasomal degradation.
Biochemical Properties The ULK1 complex core component ATG101 is originally identified as an interacting partner of ATG13 that stabilizes ATG13 within the ULK1 complex. The protruding C-terminal domain of ATG101 as a key structure for interacting with multiple class III phosphatidylinositol 3-kinase (PtdIns3K) complex components such as Beclin1, Atg14, and Vps34.
Significance in milk autophagy status in dairy cows with mild fatty liver.
PTMs Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs ATG101 Is Ubiquitinated through C-Terminal Region probably on Lys-48.HUWE1 is the primary E3 ligase targeting ATG101 and that HUWE1-mediated ubiquitination negatively regulates ATG101 protein stability.HUWE1 suppresses autophagy activity through regulating ATG101 levels,also ubiquitination of ATG101 might impact on the interaction of ATG101 and other downstream target, WIPI2.
Additional Comments Knockout of ATG101 in cancer cells using CRISPR resulted in severe growth retardation and lower survival under nutrient starvation.Atg101 stabilizes the expression of Atg13 in the cell, suggesting that Atg101 contributes to Atg13 function by protecting Atg13 from proteasomal degradation.Atg1 complex is involved in the induction of macroautophagy in mammalian cells.
Bibliography 1.Lee, J., Kim, J., Shin, J., Kang, Y., Choi, J., & Cheong, H. (2021). ATG101 Degradation by HUWE1-Mediated Ubiquitination Impairs Autophagy and Reduces Survival in Cancer Cells. International journal of molecular sciences, 22(17), 9182. 2.Mercer, C. A., Kaliappan, A., & Dennis, P. B. (2009). A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy. Autophagy, 5(5), 649-662.