Primary Information | |
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BoMiProt ID | Bomi4161 |
Protein Name | ATP-dependent DNA/RNA helicase DHX36/DEAD/H box polypeptide 36/DEAH-box protein 36/G4-resolvase-1/MLE-like protein 1/RNA helicase associated with AU-rich element protein |
Organism | Bos taurus |
Uniprot ID | Q05B79 |
Milk Fraction | Whey |
Ref Sequence ID | NP_001073720.1 |
Aminoacid Length | 1010 |
Molecular Weight | 114826 |
FASTA Sequence | Download |
Gene Name | DHX36 |
Gene ID | 509583 |
Protein Existence Status | reviewed |
Secondary Information | |
Protein Function | ATP-dependent helicase that unwinds G-quadruplex (G4) structures.Master regulator of cell homeostasis.associated with transcriptional activation of several genes involved in cell growth and differentiation by binding and resolving G4s in their respective promoters.proteins that bind ton AU rich elements(AREs) and playing role in RNA deadenylation and degradation.DHX36 controls the TP53 dependent DNA damage response, which is activated upon ultraviolet (UV) lightinduced DNA cross-linking or disruption. |
Biochemical Properties | contains specific N-terminal region which harbors a Gly-rich region and a conserved nucleic acid-binding motif (DHX36-specific motif [DSM]).N-terminal domain has two helices (α1 and α2) followed by an unstructured linker region.The N terminal is imp for enzymatic activity and subvellular localization.the core helicase portion is comprised of two RecA-like folds. These are defined by their ATPase structure of a central beta sheet surrounded by alpha helices. Within the beta strands are the conserved Walker A (GxxxxGKT/S; XXXX: variable quartett) and Walker B (hhhhD; h: hydrophobic amino acid) motifs,which coordinate the γ-phosphate of an ATP and a Mg2+ ion, respectively. |
PTMs | Acetylation and phosphorylation |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|Q05B79|DHX36_BOVIN ATP-dependent DNA/RNA helicase DHX36 OS=Bos taurus OX=9913 GN=DHX36 PE=1 SV=1 MSYDYHQNWGRDGGPRSSGGGYGGSYGGSHGGGHGGNRGSGGGGGGGGGRGGRGRHPGHL KGREIGLWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLHSVQTKNDKDEEAQISWF APEDHGYGTEAPAENKPNSVKNVEHQEKKMINQEKRPFRIRDKYIDRDSEYLLQENEPDA TLDQQLLEDLQKKKTDLRYIEMQRFREKLPSYGMQKELVNMIDNHQVTVISGETGCGKTT QVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGNGNSTGYQIRLQS RLPRKQGSILYCTTGIILQWLQSDPHLSSVSHIVLDEIHERNLQSDVLMTVVKDLLSYRP DLKVVLMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPEQKEHRSQF KKGFMQGHVNRQEKEEKEAIYKERWPGYLRELRQRYSASTVDVVEMMDDEKVDLNLIAAL IRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFIIIPLHSLMPTVNQTQVFK RTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQ RKGRAGRVQPGHCYHLYNSLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAHFLSRL MDPPSNEAVLLSIKHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPV LTIAASLSFKDPFVIPLGKEKVADARRKELAKDTKSDHLTVVNAFKGWEKAKQRGFRYEK DYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPQDPESNINSDNEKIIKAVICAG LYPKVAKIRLNLGKKRKMVKVYTKTDGVVAIHPKSVNVEQTEFNYNWLIYHLKMRTSSIY LYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIIFQSPARIAHLVKELRKELDILLQ EKIES*965PHPVDWKDTKSRDCAVLSAIIDLIKTQEKATPRNLPPRFQDGYYS |
CATH | Matched CATH superfamily n/a |
Predicted Disorder Regions | 3-70, 105-178, 819-823, |
DisProt Annotation | |
TM Helix Prediction | No TM helices |
PDB ID | 5VHA,5VHC,5VHD,5VHE, |
Bibliography | 1.Schult P, Paeschke K. The DEAH helicase DHX36 and its role in G-quadruplex-dependent processes. Biol Chem. 2020 Oct 12;402(5):581-591. doi: 10.1515/hsz-2020-0292. PMID: 33021960. 2.Chen MC, Tippana R, Demeshkina NA, Murat P, Balasubramanian S, Myong S, Ferré-D'Amaré AR. Structural basis of G-quadruplex unfolding by the DEAH/RHA helicase DHX36. Nature. 2018 Jun;558(7710):465-469. doi: 10.1038/s41586-018-0209-9. Epub 2018 Jun 13. PMID: 29899445; PMCID: PMC6261253. |