Primary Information |
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BoMiProt ID | Bomi4157 |
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Protein Name | ATP-dependent 6-phosphofructokinase, muscle type/6-phosphofructokinase type A/Phosphofructo-1-kinase isozyme A/Phosphohexokinase |
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Organism | Bos taurus |
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Uniprot ID | Q0IIG5 |
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Milk Fraction | Whey |
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Ref Sequence ID | NP_001068736.1 |
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Aminoacid Length | 779 |
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Molecular Weight | 85294 |
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FASTA Sequence |
Download |
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Gene Name | PFKM |
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Gene ID | 506544 |
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Protein Existence Status | reviewed |
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Secondary Information |
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Protein Function | Catalyzes the first committing step of glycolysis.the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP. |
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Biochemical Properties | Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.requires Mg2+ as cofactor.Bovine PFK molecular weight of the bovine native PFK was estimated to 400,000.heterotetramer composed of three kinds of subunit with molecular weights of 92,000 (C-type), 88,000 (M-type) and 86,000 (L-type). |
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PTMs | Acetylation, Glycosylation, Hydroxylation, Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q0IIG5|PFKAM_BOVIN ATP-dependent 6-phosphofructokinase, muscle type OS=Bos taurus OX=9913 GN=PFKM PE=2 SV=1
MTHEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIYTGARVFFVHEGYQGLVDG
GDNIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGS
LTGADTFRSEWS*132DLLSDLQKSGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSA
LHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEE
HLCRRLSETRNRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQR
GGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTRA
MDERRFDEALKLRGRS*376FMNNWEVYKLLAHVRPPKSKSGSHTVAVMNVGAPAAGMNAAVRS
TVRIGLIQGNRMLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQI
SANITKFNIQGLVIIGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVS*529NNVPGSDFSVG
ADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEDPFT
IRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGH
MQQGGS*666PTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANSPDSGCVLGMRKRALVFQ
PVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRS*774GEANV
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Predicted Disorder Regions | NA |
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DisProt Annotation | |
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TM Helix Prediction | No TM helices |
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Bibliography | Fukushima E, Sugiya H. Purification and characterization of phosphofructokinase in bovine parotid gland. Int J Biochem. 1992 Aug;24(8):1307-14. doi: 10.1016/0020-711x(92)90206-g. PMID: 1386580. |