Primary Information |
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BoMiProt ID | Bomi3986 |
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Protein Name | Arf-GAP domain and FG repeat-containing protein 1/HIV-1 Rev-binding protein homolog/Nucleoporin-like protein RIP |
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Organism | Bos taurus |
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Uniprot ID | Q2TA45 |
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Milk Fraction | Whey |
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Ref Sequence ID | NP_001033260.1 |
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Aminoacid Length | 562 |
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Molecular Weight | 58134 |
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FASTA Sequence |
Download |
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Gene Name | AGFG1/HRB/RIP |
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Gene ID | 536149 |
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Protein Existence Status | reviewed |
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Secondary Information |
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Protein Function | They control the activity of the GTPbinding proteins of the ARF family by inducing the hydrolysis of GTP that is bound to Arf proteins.It also plays a role in vesicle formation. |
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Biochemical Properties | ARF family do not have detectable intrinsic GTPase activity, the complex of the Arf GAP with Arf1 could be viewed as the functional GTPase, which essentially would consist of a heterodimer. The functional heterodimer is probably not very stable and may dissociate right after catalysis. Taken together, this indicates that specificity for Arf GTP-binding proteins is mostly dominated by Arf GAPs. Moreover, most Arf effector molecules interact with the GTP-bound form of Arf. Thus Arf GAPs may play a critical role in terminating Arf-effector protein interactions.Arf GAP was identified in yeast, Gcs1p, which is the homologue of mammalian Arf GAP1. |
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PTMs | O-Linked Glycosylation at Ser,Phosphorylation at Ser/Thr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q2TA45|AGFG1_BOVIN Arf-GAP domain and FG repeat-containing protein 1 OS=Bos taurus OX=9913 GN=AGFG1 PE=2 SV=1
MAASAKRKQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLN
PPHRVKSISMTTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEK
YEKKRWYVPPEQAKVVASVHASISGSSASSTSSTPEVKPLKSLLGDS*167APALHLNKGT*177PSQ
S*181PVVGRSQAQQQEKKQFDLLSDLGSDIFAAPAPQSTATANFANFAHFNSHAAQNSANADF
ANFDAFGQSSGSSNFGGFPTASHSSFQPQTTGGSAGSVNANFAHFDNFPKSSSADFGTFN
TSQSHQTASAVSKVSANKAGLQTTDKYAALANLDNIFSAGQGGDQGSGFGTTGKAPVGSV
VS*362VPSQS*367SASSDKYAALAELDSVFSSAATSSNAYTSTSNASSNVFGTVPVGASAQTQPAS
SSVPAPFGATPSTNPFVAAAGPSVASSTNPFQTNARGATAATFGTASMSMPAGFGTPAPY
SLPTSFSGSFQQPAFPAQAAFPQQTAFSQQPNGAGFAAFGQTKPVVTPFGQVGAAGVSSN
PFMTGAPTGQFPTGSSSTNPFL
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Predicted Disorder Regions | 1-13, 144-329, 340-562 |
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DisProt Annotation | |
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TM Helix Prediction | No TM helices |
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Additional Comments | Thirty-one genes encoding proteins with Arf GAP catalytic domains have been identified in humans. Ten subfamilies have been described based on domain structure and phylogenetic analysis. Six subfamilies have Arf GAP domains at the extreme N-terminus. Four subfamilies (ASAP, ACAP, AGAP and ARAP), comprising 20 proteins, have a catalytic core of PH, Arf GAP and ANK repeat domains. At least one member of eight subtypes has confirmed GAP activity. |
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Bibliography | Anne Spang; Yoko Shiba; Paul A. Randazzo (2010). Arf GAPs: Gatekeepers of vesicle generation. , 584(12), 2646–2651. doi:10.1016/j.febslet.2010.04.005 |