Primary Information |
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| BoMiProt ID | Bomi3848 |
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| Protein Name | ALS2 C-terminal-like protein |
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| Organism | Bos taurus |
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| Uniprot ID | A6QP75 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001095958.1 |
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| Aminoacid Length | 953 |
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| Molecular Weight | 107869 |
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| FASTA Sequence |
Download |
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| Gene Name | ALS2CL |
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| Gene ID | 506969 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosome dynamics.ALS2CL is a novel factor modulating the Rab5-mediated endosome dynamics in the cells. |
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| Biochemical Properties | Analysis of the predicted amino acid sequences revealed a high level of sequence similarity throughout the entire region of ALS2CL and the C-terminal portion of the ALS2 proteins, indicating that ALS2CL/Als2cl and ALS2/Als2 were evolutionally conserved genes evolved from a common ancestry of origin. |
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| PTMs | N6-acetylation of Lys-44, Phosphorylation at many AA residues, Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| na |
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Autophosphorylation at Thr-186, Ser-347, Thr-350, Ser-353, Thr-354 and Ser-357 triggers kinase activity by promoting cyclin and substrate binding upon conformational changes.Phosphorylation at Thr-186 requires the calcium Ca2+ signaling pathway, including CaMK1D and calmodulin.N6-acetylation of Lys-44 promotes kinase activity, whereas acetylation of both Lys-44 and Lys-48 mediated by PCAF/KAT2B and GCN5/KAT2A reduces kinase activity. |
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| Additional Comments | ALS2 may implicate in the vesicle/membrane dynamics in the cells through the activation of Rab5. |
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| Bibliography | Hadano S, Otomo A, Suzuki-Utsunomiya K, Kunita R, Yanagisawa Y, Showguchi-Miyata J, Mizumura H, Ikeda JE. ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics. FEBS Lett. 2004 Sep 24;575(1-3):64-70. doi: 10.1016/j.febslet.2004.07.092. PMID: 15388334. |
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