Search by BoMiProt ID - Bomi3843


Primary Information

BoMiProt ID Bomi3843
Protein Name Alpha-N-acetylgalactosaminidase/Alpha-galactosidase B
Organism Bos taurus
Uniprot IDQ58DH9
Milk FractionWhey
Ref Sequence ID NP_001039814.1
Aminoacid Length 411
Molecular Weight 46533
FASTA Sequence Download
Gene Name NAGA
Gene ID 533357
Protein Existence Status reviewed

Secondary Information

Protein Function  Enzymatical conversion of A RBCs into group O RBCs (ECORBCs) was achieved by using α-N-acetylgalactosaminidase.
Biochemical Properties The marine invertebrate starfish was found to contain α-N-acetylgalactosaminidase, α-GalNAcase II, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc), in addition to a typical α-N-acetylgalactosaminidase, α-GalNAcase I, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc) and, to a lesser extent, galactose. 
PTMs Disulfide bond formation,N-linked Glycosylation at Asn, Phosphorylation at Ser
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q58DH9|NAGAB_BOVIN Alpha-N-acetylgalactosaminidase OS=Bos taurus OX=9913 GN=NAGA PE=2 SV=1 MLLKTVLLLALASQVLVLENGLLRKPPMGWLAWERFRCNIDCSEDPKNCISEQLFMEMAD RLAQDGWRDLGYVYLNIDDCWIGGRDAKGNLVPDRKRFPHGIAFLADYAHSLGLKLGIYE DLGN*124FTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCYSTPQERAEGYPKMAAALN*177ATG RPIAFSCSWPAYEGGLPPKVN*201YTLLADICNLWRNFDDIQDSWRSVLSVLDWFVTHQDVLQ PIAGPGHWNDPDMLLIGNFGLSFEQAQAQMALWTVLAAPLFMSTDLRTISAQNMDILQNP LMIKINQDPLGIQGRRILKEKS*322HIEVYLRPLASEASAIVFFSRRMDMPYHYHSSLARLN*359F SSSVVYEAQDVYTGDIISGLQDKTN*385FTVIINPSGVVMWYLYPIRKLEIPQQ
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments α-N-acetylgalactosaminidase (nagalase) facilitates the deglycosylation of Gc-MAF, which in turn inhibits the activation of macrophages.
Bibliography 1.Rashid MH, Sadik G, Alam AK, Tanaka T. Chemical and structural characterization of α-N-acetylgalactosaminidase I and II from starfish, asterina amurensis. BMC Biochem. 2017 May 25;18(1):9. doi: 10.1186/s12858-017-0085-1. PMID: 28545388; PMCID: PMC5445309. 2.Saburi E, Tavakol-Afshari J, Biglari S, Mortazavi Y. Is α-N-acetylgalactosaminidase the key to curing cancer? A mini-review and hypothesis. J BUON. 2017 Nov-Dec;22(6):1372-1377. PMID: 29332325. 3.Gao H, Li S, Tan Y, Ji S, Wang Y, Bao G, Xu L, Gong F. Application of α-N-acetylgalactosaminidase and α-galactosidase in AB to O red blood cells conversion. Artif Cells Nanomed Biotechnol. 2013 Feb;41(1):32-6. doi: 10.3109/10731199.2012.724422. Epub 2012 Oct 2. PMID: 23030311.