Primary Information |
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| BoMiProt ID | Bomi3701 |
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| Protein Name | Adenylyl cyclase-associated protein 1 |
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| Organism | Bos taurus |
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| Uniprot ID | Q3SYV4 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001030182.1 |
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| Aminoacid Length | 472 |
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| Molecular Weight | 51273 |
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| FASTA Sequence |
Download |
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| Gene Name | CAP1 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Highly conserved in evolution.Bifunctional protein involved in Ras-adenylyl cyclase and F-actin dynamics regulation. |
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| Biochemical Properties | conserved RLE motif in mammalian CAP1 interacts with other coiled-coil–containing proteins.CAP1 can be separated into two subdomains: a helical N-terminal (1 to 318) and a β-sheet C-terminal (319 to 475) subdomain. |
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| Significance in milk | is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells |
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| PTMs | Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|Q3SYV4|CAP1_BOVIN Adenylyl cyclase-associated protein 1 OS=Bos taurus OX=9913 GN=CAP1 PE=2 SV=3
MADMRNLVERLERVVGRLEAVSHASDTHCGY*31GDS*34AAKAGTTPYVQAFDSLLAGPVAEYLK
ISKEIGGDVQKHAEMVHTGLKLERALLVTASQCQQPAGNKLSDLLAPISEQIQEVVTFRE
KNRGSKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHV
DWVKAYLSIWTELQAYIKEFHTTGLAWSRTGPVAKELSGLPSGPSAGSGPPPPPPGPPPP
PVPTSSGSDDSASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQS*290GLIRS*295GPKPF
S*301ASKPDPPKPVAKKEPALLELEGKKWRVENQENVSNLMIEDTELKQVAYIFKCVNSTLQI
KGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHVYLSKN
SLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG
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| Predicted Disorder Regions | 29-39, 210-253, 270-314 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | phosphorylated S308 and S310 in CAP1 promoted lung cancer cell proliferation, migration, and metastasis both in vitro and in vivo. When de-phosphorylated, these two sites in CAP1 showed the opposite effect. Phosphorylation of CAP1 can promote epithelial–mesenchymal transition (EMT). |
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| Bibliography | Zeng, J., Li, X., Liang, L. et al. Phosphorylation of CAP1 regulates lung cancer proliferation, migration, and invasion. J Cancer Res Clin Oncol 148, 137–153 (2022). https://doi.org/10.1007/s00432-021-03819-9 |
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