Search by BoMiProt ID - Bomi3698

Primary Information

BoMiProt ID Bomi3698
Protein Name Adenylate kinase 2, mitochondrial/ATP-AMP transphosphorylase 2/Adenylate monophosphate kinase
Organism Bos taurus
Uniprot IDP08166
Milk FractionWhey
Ref Sequence ID NP_776314.1
Aminoacid Length 241
Molecular Weight 26497
FASTA Sequence Download
Gene Name AK2
Gene ID 280716
Protein Existence Status Reviewed

Secondary Information

Presence in other biological fluids/tissue/cells  rumen epithelium
Protein Function Adenylate kinase 2 (AK2), a mitochondrial enzyme that regulates adenine nucleotide interconversion within the intermembrane space, is markedly induced during adipocyte and B cell differentiation.Molecular studies indicate that AK2 participates in cell fate decisions and nuclear energetics.
Biochemical Properties Catalyses the following reaction, AMP + ATP = 2 ADP.Typical AK equilibrium ([ADP]2/[ATP]·[AMP]) and Michaelis (Km for ADP) constants are 2.26 and 1.58 mmol l−1 respectively.
PTMs N-Acetylation at Meth, Disulfide bond formation,Phosphorylation at Ser
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
SCOP Class : Alpha and beta proteins (a+b)
Fold : P-loop kinase-like
Superfamily : P-loop nucleotide/nucleoside kinase-like
Family : Adenylate kinase-like
Domain Name : 1AK2 A:14-232

CATH Matched CATH superfamily
Predicted Disorder Regions 1-17, 60-62, 152-169
DisProt Annotation
TM Helix Prediction No TM helices
PDB ID 1AK2, 2AK2,
Additional Comments AK2 mutations have been associated with rare congenital human diseases linked to early mortality, immune deficiency and hearing loss.Depletion of AK2 by RNAi impairs adiponectin secretion in 3T3-L1 adipocytes, IgM secretion in BCL1 cells, and the induction of the UPR during differentiation of both cell types. The enzyme has gained much attention recently because it can be used as a cell marker to replace ATP in the luciferase-based bioluminescence assay.
Bibliography 1.Burkart A, Shi X, Chouinard M, Corvera S. Adenylate kinase 2 links mitochondrial energy metabolism to the induction of the unfolded protein response. J Biol Chem. 2011 Feb 11;286(6):4081-9. doi: 10.1074/jbc.M110.134106. Epub 2010 Sep 27. PMID: 20876536; PMCID: PMC3039340. 2.Zhang S, Yamada S, Park S, Klepinin A, Kaambre T, Terzic A, Dzeja P. Adenylate kinase AK2 isoform integral in embryo and adult heart homeostasis. Biochem Biophys Res Commun. 2021 Mar 26;546:59-64. doi: 10.1016/j.bbrc.2021.01.097. Epub 2021 Feb 8. PMID: 33571905; PMCID: PMC7939106.