Search by BoMiProt ID - Bomi366

Primary Information

BoMiProt ID Bomi366
Protein Name Asparaginyl-tRNA synthetase, cytoplasmic
Organism Bos taurus
Uniprot IDQ2KJG3
Milk FractionExosome
Ref Sequence ID NP_001040037.1
Aminoacid Length 559
Molecular Weight 64399
FASTA Sequence Download
Gene Name NARS
Gene ID 616033
Protein Existence Status Reviewed: Experimental evidence at transcript level

Secondary Information

Protein Function enzymes involved in protein biosynthesis catalyzing the specific attachment of amino acids to their cognate tRNAs. Aminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. 
Biochemical Properties classified as a sub-class IIb enzyme together with the aspartyl- and lysyl-enzymes on the basis of similarities in their N-terminal extensions and the catalytic domains; absence of a mitochondrial import signal. The N-terminal domain contains a structured region with a novel fold featuring a lysine-rich helix that is shown by NMR to interact with tRNA.
Significance in milk might play a part in tissue differentiation and regulation of mammary gland; individual tRNA's and aminoacyl-tRNA synthetases correspond to the amino acid composition of the main milk proteins
PTMs N6-acetylation at Lysine,Phosphorylation at Serine
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Bibliography 1. Beaulande, M., Tarbouriech, N. and Hartlein, M. (1998) ‘Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence, functional expression in Escherichia coli and characterization as human autoantigen’, Nucleic Acids Research, 26(2), pp. 521–524. doi: 10.1093/nar/26.2.521.
2. Cusack, S., Härtlein, M. and Leberman, R. (1991) ‘Sequence, structural and evolutionary relationships between class 2 aminoacyl-tRNA synthetases’, Nucleic Acids Research, 19(13), pp. 3489–3498. doi: 10.1093/nar/19.13.3489. 3.Crepin T, Peterson F, Haertlein M, Jensen D, Wang C, Cusack S, Kron M. A hybrid structural model of the complete Brugia malayi cytoplasmic asparaginyl-tRNA synthetase. J Mol Biol. 2011 Jan 28;405(4):1056-69. doi: 10.1016/j.jmb.2010.11.049. Epub 2010 Dec 4. PMID: 21134380.