Primary Information |
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| BoMiProt ID | Bomi365 |
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| Protein Name | Serine/threonine-protein kinase 25 |
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| Organism | Bos taurus |
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| Uniprot ID | Q3SWY6 |
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| Milk Fraction | MFGM |
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| Ref Sequence ID | NP_899666.1 |
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| Aminoacid Length | 426 |
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| Molecular Weight | 48035 |
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| FASTA Sequence |
Download |
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| Gene Name | STK25 |
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| Gene ID | 373543 |
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| Protein Existence Status | Reviewed: Experimental evidence at transcript level |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | As found in rats, expression was highest in
the small and large intestine and the brain, compared with
heart, skeletal muscle, spleen, liver, lung, kidney and
adipose tissues; |
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| Protein Function | Might be involved in the regulation of a novel
intracellular signalling pathway; regulation
of cell migration and modulation of cell death; pays a role in determining the proper localisation
and morphology of the Golgi complex: STK25 inhibition
perturbs perinuclear Golgi
organisation, cell migration and invasion into type I collagen |
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| Biochemical Properties | STK25 has been shown to be slightly activated by reactive oxygen intermediates but unaffected by growth factors, heat shock or osmolar stress; interact with
diverse substrates ranging from enzymes to transcription factors, receptors, and other regulatory proteins; associate with the Golgi matrix proteins- Golgi
matrix (GM)130 and binding to GM130 was shown to activate the
kinase by promoting autophosphorylation of STK25 |
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| Significance in milk | increase in protein supports milk protein synthesis during the periparturient period |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Predicted Disorder Regions | (292-355) |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Bibliography | 1. Ma, Y. F. et al. (2019) ‘Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows’, Journal of Dairy Science, 102(5), pp. 4264–4274. doi: 10.3168/jds.2018-15451.
2. Nerstedt, A. et al. (2012) ‘Serine/threonine protein kinase 25 (STK25): a novel negative regulator of lipid and glucose metabolism in rodent and human skeletal muscle’, Diabetologia, 55(6), pp. 1797–1807. doi: 10.1007/s00125-012-2511-7.
3. Kostich, M. et al. (2002) ‘Human members of the eukaryotic protein kinase family.’, Genome Biology, 3(9), p. research0043.1. doi: 10.1186/gb-2002-3-9-research0043. |
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