Primary Information
BoMiProt ID	Bomi3628
Protein Name	Actin-related protein 8
Organism	Bos taurus
Uniprot ID	Q1LZF2
Milk Fraction	Whey
Ref Sequence ID	NP_001069829.1
Aminoacid Length	624
Molecular Weight	70485
FASTA Sequence	Download
Gene Name	ACTR8/ARP8
Gene ID	615133
Protein Existence Status	reviewed
Secondary Information
Protein Function	Arp4, assisted by Arp8, influences actin polymerization in vitro and is able to depolymerize actin filaments.The another contribution of Arp8 is enhancing the nucleosome-binding ability of INO80 is significant.
Biochemical Properties	Actin, Arp4 and Arp8 together with the helicase-SANTassociated (HSA) domain of the Ino80 ATPase form a stable sub-module of INO80, while Arp5 together with INO80 subunits Ies2 and Ies6 associate with the AAA+ ATPase subunits Rvb1/Tip49 and Rvb2/Tip48.
PTMs	N-Linked Acetylation at Meth,Phosphorylation at Ser
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q1LZF2|ARP8_BOVIN Actin-related protein 8 OS=Bos taurus OX=9913 GN=ACTR8 PE=2 SV=1 MTQAEKGEAENGKEKGGEKEKEQRGVKRPIVPALVPESLQEQIQSNFIVVIHPGSTTLRI GRATDTLPASVPHVIARRHKQQGQPLYKDSWLLREGLNKPESNEQRQNGLKMVDQAIWSK KMSNGTRRIPVS*132PEQARSYNKQMRPAILDHCSGNKWTNTSHHPEFLVGEEALYVNPLDCY NIHWPIRRGQLNIHPGPGGSLTAVLADIEVIWSHAIQKYLEIPLKDLKYYRCILLIPDIY NKQHVKELVNMILMKMGFSGIVVHQESVCATFGSGLSSTCIVDVGDQKTSVCCVEDGVSH RNTRLCLAYGGSDVSRCFYWLMQRAGFPYRECQLTNKMDCLLLQHLKETFCHLDQDISGL QDHEFQIRHPDSPALLYQFRLGDEKLQAPMALFYPATFGIVGQKMTTLQHRS*412QGDPEDPH DEQYLLATQSKQEQSAKATADRKSASKPIGFEGDLRGQSSDLPERLHSQEVDLGPSQGDC LMAGNDSEEALTALMSRKTAISLFEGKALGLDKAILHSIDCCSSDETKKKMYSSILVVGG GLMFHKAQEFLQHRILNKMPPSFRRIIENVDVITRPKDMDPRLIAWKGGAVLACLDTTQE LWIYQREWQRFGVRMLRERAAFVW
Predicted Disorder Regions	1-40, 83-132, 416-476
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	Deleting Arp8 in Saccharomyces cerevisiae results in an INO80 complex that also lacks Arp4 and actin, in addition to the losses of DNA binding and ATPase activities
Bibliography	1.. Szerlong,H., Hinata,K., Viswanathan,R., Erdjument-Bromage,H., Tempst,P. and Cairns,B.R. (2008) The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases. Nat. Struct. Mol.Biol., 15, 469–476. 2.Chen,L., Cai,Y., Jin,J., Florens,L., Swanson,S.K., Washburn,M.P., Conaway,J.W. and Conaway,R.C. (2011) Subunit organization of the human INO80 chromatin remodeling complex. J. Biol. Chem., 286, 11283–11289. 3.. Shen,X., Ranallo,R., Choi,E. and Wu,C. (2003) Involvement of actin-related proteins in ATP-dependent chromatin remodeling. Mol. Cell, 12, 147–155. 4.Fenn S, Breitsprecher D, Gerhold CB, Witte G, Faix J, Hopfner KP. Structural biochemistry of nuclear actin-related proteins 4 and 8 reveals their interaction with actin. EMBO J. 2011 Jun 1;30(11):2153-66. doi: 10.1038/emboj.2011.118. Epub 2011 Apr 15. PMID: 21499228; PMCID: PMC3117639.