Primary Information | |
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BoMiProt ID | Bomi3620 |
Protein Name | Aconitate hydratase, mitochondrial/Citrate hydro-lyase |
Organism | Bos taurus |
Uniprot ID | P20004 |
Milk Fraction | Whey |
Ref Sequence ID | NP_776402.1 |
Aminoacid Length | 780 |
Molecular Weight | 85359 |
FASTA Sequence | Download |
Gene Name | ACO2 |
Gene ID | 280976 |
Protein Existence Status | Reviewed |
Secondary Information | |
Presence in other biological fluids/tissue/cells | heart |
Protein Function | Mitochondrial aconitase (ACO2) is involved in the TCA cycle and catalyzes the isomerization of citrate to isocitrate via cis-aconitate, which associates with ATP-dependent sperm motility. |
Biochemical Properties | Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Binds 1 [4Fe-4S] cluster per subunit. Binding of a [3Fe-4S] cluster leads to an inactive enzyme.Highly sensitive to irreversible oxidative inactivation by H2O2.The catalytic residues involved are His-101 and Ser-642. |
PTMs | N6-acetylation at Lysine,Phosphorylation at Ser |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial OS=Bos taurus OX=9913 GN=ACO2 PE=1 SV=4 MAPYSLLVSRLQKALGARQYHVASVLCQRAKVAMSHFEPNEYIRYDLLEKNINIVRKRLN RPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAV PSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILEN YAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSG WTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYN HRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDLAHP VAEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPGS EQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRN DANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPG QDTYQHPPKDSSGQQVDVS*559PTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAA GPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVI GDENYGEGSS*670REHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHP VDKLTIKGLKDFAPGKPLTCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQK |
SCOP | Class : Alpha and beta proteins (a/b) Fold : Aconitase iron-sulfur domain Superfamily : Aconitase iron-sulfur domain Family : Aconitase iron-sulfur domain Domain Name : 1ACO A:2-528 Class : Alpha and beta proteins (a/b) Fold : The swivelling beta/beta/alpha domain Superfamily : LeuD/IlvD-like Family : LeuD-like Domain Name : 1ACO A:529-753 |
CATH | Matched CATH superfamily 3.20.19.10 3.30.499.10 3.40.1060.10 |
Predicted Disorder Regions | 532-571 |
DisProt Annotation | |
TM Helix Prediction | No TM helices |
Significance of PTMs | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers. |
PDB ID | 1ACO, 1AMI, 1AMJ, 1C96, 1C97, 1FGH, 1NIS, 1NIT, 8ACN, |
Additional Comments | low expression of ACO2 might result in low production of isocitrate negatively affecting the TCA cycle to eventually disrupt most sperm motility. |
Bibliography | 1.Tang M, Liu BJ, Wang SQ, Xu Y, Han P, Li PC, Wang ZJ, Song NH, Zhang W, Yin CJ. The role of mitochondrial aconitate (ACO2) in human sperm motility. Syst Biol Reprod Med. 2014 Oct;60(5):251-6. doi: 10.3109/19396368.2014.915360. Epub 2014 Apr 30. PMID: 24785945. 2.Beinert, H. and Kennedy, M.C. (1993) Aconitase, a two-faced protein: enzyme and iron regulatory factor. FASEB J 7:1442–9. 3.Wang, S., Wang, W., Xu, Y., Tang, M., Fang, J., Sun, H., et al. (2014) Proteomic characteristics of human sperm cryopreservation. Proteomics 14: 298--310. |