Primary Information |
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| BoMiProt ID | Bomi335 |
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| Protein Name | 6-phosphogluconate dehydrogenase, decarboxylating |
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| Organism | Bos taurus |
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| Uniprot ID | Q3ZCI4 |
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| Milk Fraction | Whey, MFGM, Exosome |
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| Ref Sequence ID | NP_001137210.1 |
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| Aminoacid Length | 483 |
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| Molecular Weight | 53077 |
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| FASTA Sequence |
Download |
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| Gene Name | PGD |
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| Gene ID | 514939 |
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| Protein Existence Status | Unreviewed: Experimental evidence at transcript level |
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Secondary Information |
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| Protein Function | enzyme of the pentose phosphate pathway, providing the necessary
compounds of NADPH for the synthesis of fatty acids; oxidative
carboxylase that catalyzes the decarboxylating reduction of 6-
phosphogluconate into ribulose 5-phosphate in the presence
of NADP; functional importance of the enzyme is in providing NADPH for fat synthesis and ribose
for nucleic acid synthesis; |
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| Biochemical Properties | The enzyme follows a rapid equilibrium bi-ter sequential mechanism, with a random order of substrate addition both in the direct reaction, oxidative decarboxylation, and, in the reverse reaction, reductive carboxylation; substrate of reductive carboxylation is ribulose-5-phosphate; 6PG at low concentrations decreases the Km of Ru5P; 6PG is like an allosteric activator of 6PGDH |
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| Significance in milk | As found in ovine, 6PGD activity in tail adipose tissue
is reduced with the increase of milk energy; In ewes with high milk production, the pathway
of lipogenesis is limited by reducing the size of fat cells
and reducing the activity of 6PGD |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Bibliography | 1. Hanau, S. et al. (2010) ‘6-Phosphogluconate Dehydrogenase Mechanism’, Journal of Biological Chemistry, 285(28), pp. 21366–21371. doi: 10.1074/jbc.M110.105601.
2. Trivizaki, S. et al. (2010) ‘Enzymatic and mRNA Transcript Response of Ovine 6-Phosphogluconate Dehydrogenase (6PGD) in Respect to Different Milk Yield.’, Biochemistry research international. Hindawi, 2010, p. 512056. doi: 10.1155/2010/512056. |
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