Search by BoMiProt ID - Bomi322

Primary Information

BoMiProt ID Bomi322
Protein Name CD47 molecule
Organism Bos taurus
Uniprot IDQ08DW0
Milk FractionMFGM, Exosome
Ref Sequence ID NP_777133.2
Aminoacid Length 303
Molecular Weight 33311
FASTA Sequence Download
Gene Name CD47
Gene ID 282661
Protein Existence Status Unreviewed: Experimental evidence at transcript level

Secondary Information

Presence in other biological fluids/tissue/cells virtually all cells in the body
Protein Function integral membrane protein that serves as the counter-receptor for the inhibitory phagocyte receptor signal-regulatory protein-α (SIRPα) and as a signaling receptor for the secreted matricellular protein thrombospondin-1; important pathophysiological functions of CD47 in cardiovascular homeostasis, immune regulation, resistance of cells and tissues to stress, and chronic diseases of aging including cancer; can regulate many important physiological cellular mechanisms by interacting with integrins, TSP-1, or SIRPα
Biochemical Properties lacks a substantial cytoplasmic signaling domain, but several cytoplasmic binding partners have been identified; N-terminal extracellular IgV domain derived from the immunoglobulin superfamily followed by a presenilin domain containing five membrane-spanning segments and ending in a short variably spliced cytoplasmic sequence; disulfide bond links Cys33 in the IgV domain to Cys263 in the last extracellular loop in the transmembrane domain and is essential for some signaling functions of CD47
Significance in milk negatively regulate phagocytosis of apoptotic mammary epithelial cells by bystander epithelial cells during mammary gland involution
PTMs Glycosylated: homotrimeric glycoprotein;IgV domain is N-glycosylated and modified with an O-linked glycosaminoglycan
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Significance of PTMs is required for thrombospondin-1 (TSP1) signaling through CD47
Bibliography 1. Rebres, R. A. et al. (2001) ‘Membrane Raft Association of CD47 Is Necessary for Actin Polymerization and Protein Kinase C θ Translocation in Its Synergistic Activation of T Cells’, Journal of Biological Chemistry, 276(10), pp. 7672–7680. doi: 10.1074/jbc.M008858200.
2. Kaur, S. et al. (2011) ‘Heparan Sulfate Modification of the Transmembrane Receptor CD47 Is Necessary for Inhibition of T Cell Receptor Signaling by Thrombospondin-1’, Journal of Biological Chemistry, 286(17), pp. 14991–15002. doi: 10.1074/jbc.M110.179663.