Primary Information
BoMiProt ID	Bomi32
Protein Name	Fetuin
Organism	Bos taurus
Uniprot ID	Q58D62
Milk Fraction	Whey
Ref Sequence ID	NP_001029390.1
Aminoacid Length	387
Molecular Weight	42663
FASTA Sequence	Download
Gene Name	FETUB
Gene ID	504615
Protein Existence Status	Reviewed: Experimental evidence at protein level
Secondary Information
Presence in other biological fluids/tissue/cells	Identified primarily in blood and brain
Protein Function	member of the cystatin superfamily; identified as major proteins during fetal life; mRNAlevel is down-regulated during the acute phase of experimentally induced inflammation in rat; opsonize cationic macrophage-deactivating molecules;
Biochemical Properties	homologous to fetuin-A structurally; Nonspecific hydrophobic interactions were supposed to play a central role in maintaining a hydrophobic core, around which are held together the secondary structural elements and the fluctuating side chains of the protein; potential stabilizing factor for the stability of the molten globule state of BSF is the presence of disulfide bonds
Significance in milk	Involved in host defence related responses in bovine milk during an experimentally induced Streptococcus uberis infection
PTMs	complex triantennary oligosaccharides; possesses considerable N-glycosylation site heterogeneity; asialo-fetuin contains two variant triantennary oligosaccharides with either Galß(l - 3)^3 or Galß(1 - 4) at one of the terminal branch positions; presence of sialylation heterogeneity among each of these oligosaccharides; contains 12 cysteines and makes 6 disulfide bonds
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q58D62|FETUB_BOVIN Fetuin-B OS=Bos taurus OX=9913 GN=FETUB PE=1 SV=1 MNVLLLLVLCTLAMGCGATSPPQPAARPSSLLSLDCNSSYVLDIANDILQDINRDRKDGY VLSLNRVSDAREHRQEAGLGSLFYFTLDVLETGCHVLSRTSWKNCEVRIFHESVYGQCKA IFYINKEKRIFYLPAYNCTLRPVSQSAIIMTCPDCPSTSPYDLSNPRFMETATESLAKYN SESPSKQYSLVKITKTSSQWVFGPAYFVEYLIKESPCVKSEGSSCALESPGSVPVGICHG SLGEPQGNQGKIISVTCSFFNSQAPTPRGENATVNQRPANPSKTEELQQQNT*292APT*295NSPTK AVPKGSVQYLPDWDKKREGSQEKDPVETFPVQLDLTTNPQGESLDVSFLFQEPMEEKVVV LPFPSKEQRSAECPGPAQKGYPFILPS
Predicted Disorder Regions	264-306,366-387
DisProt Annotation
TM Helix Prediction	No TM helices
Additional Comments	the fetuin family encompasses a series of tightly related proteins that are synthesized mostly in the liver and have been variably designated fetuin in sheep, pig and cow, a2-HS-glycoprotein (AHSG) in human, phosphoprotein of 63 kDa (pp63) in rat, or countertrypin in mouse and gerbil
Bibliography	1. Rice, K. G., Rao, N. B. N., & Lee, Y. C. (1990). Large-scale preparation and characterization of N-linked glycopeptides from bovine fetuin. Analytical Biochemistry, 184(2), 249–258. https://doi.org/10.1016/0003-2697(90)90676-Z. 2. Smolenski, G. A., Broadhurst, M. K., Stelwagen, K., Haigh, B. J., & Wheeler, T. T. (2014). Host defence related responses in bovine milk during an experimentally induced Streptococcus uberis infection. Proteome Science, 12(1). https://doi.org/10.1186/1477-5956-12-19. 3. Olivier, E., Soury, E., Ruminy, P., Husson, A., Parmentier, F., Daveau, M., & Salier, J. P. (2000). Fetuin-B, a second member of the fetuin family in mammals. The Biochemical Journal, 350 Pt 2, 589–597. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10947975. 4 Wang, C., Lascu, I., & Giartosio, A. (1998). Bovine serum fetuin is unfolded through a molten globule state. Biochemistry, 37(23), 8457–8464. https://doi.org/10.1021/bi9723010.