Primary Information |
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| BoMiProt ID | Bomi310 |
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| Protein Name | Sodium-dependent phosphate transport protein 2B |
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| Organism | Bos taurus |
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| Uniprot ID | Q27960 |
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| Milk Fraction | Whey, MFGM, Exosome |
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| Ref Sequence ID | NP_777086.1 |
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| Aminoacid Length | 693 |
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| Molecular Weight | 75826 |
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| FASTA Sequence |
Download |
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| Gene Name | SLC34A2 |
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| Gene ID | 282484 |
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| Protein Existence Status | Reviewed: Experimental evidence at protein level |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | renal epithelial cells, small intestine |
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| Protein Function | renal Pi reabsorption; involved in Pi homeostasis; type 2b Na-dependent
phosphate co-transporter (NaPi-2b) is the main
mediator of intestinal active Pi absorption |
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| Biochemical Properties | Na+ interacts in a cooperative way with the transporter with a
stoichiometry of 3Na+ to 1Pi; Protons decreased the affinity of the transporter for Na+,
resulting in the characteristic pH-dependency of Na+/Pi
reabsorption; as found in bovine renal epithelial cell lines, presence of at least eight regions of hydrophobicity; |
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| Significance in milk | nutrient transport systems for milk precursors and constituents |
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| PTMs | Glycosylated:possible sites for N-glycosylation and phosphorylation
by protein kinase C as found in bovine renal epithelial cells; |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Predicted Disorder Regions | (1-46) |
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| DisProt Annotation | |
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| TM Helix Prediction | 11TMHs; (103-125),(138-160),(179-201),(221-239),(361-383),(406-428),(432-450),(457-475),(484-506),(527-549),(553-571) |
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| Bibliography | 1. Busch, A. et al. (1994) ‘Electrophysiological analysis of Na+/Pi cotransport mediated by a transporter cloned from rat kidney and expressed in Xenopus oocytes.’, Proceedings of the National Academy of Sciences, 91(17), pp. 8205–8208. doi: 10.1073/pnas.91.17.8205.
2. Hayes, G. et al. (1994) ‘Role of N-linked glycosylation in rat renal Na/Pi-cotransport.’, The Journal of biological chemistry, 269(39), pp. 24143–9. Available at: http://www.ncbi.nlm.nih.gov/pubmed/7929070 (Accessed: 4 October 2019).
3. Helps, C., Murer, H. and McGivan, J. (1995) ‘Cloning, Sequence Analysis and Expression of the cDNA Encoding a Sodium-Dependent Phosphate Transporter from the Bovine Renal Epithelial Cell Line NBL-1’, European Journal of Biochemistry, 228(3), pp. 927–930. doi: 10.1111/j.1432-1033.1995.tb20341.x.
4. Giral, H. et al. (2012) ‘NHE3 Regulatory Factor 1 (NHERF1) Modulates Intestinal Sodium-dependent Phosphate Transporter (NaPi-2b) Expression in Apical Microvilli’, Journal of Biological Chemistry, 287(42), pp. 35047–35056. doi: 10.1074/jbc.M112.392415. |
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