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Primary Information | |
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| BoMiProt ID | Bomi286 |
| Protein Name | Coagulation factor IX (Fragment) |
| Organism | Bos taurus |
| Uniprot ID | P00741 |
| Milk Fraction | Whey |
| Aminoacid Length | 462 |
| Molecular Weight | 52046 |
| FASTA Sequence | Download |
| Gene Name | F9 |
| Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
| Protein Function | plasma glycoprotein which participates in the middle phase of intrinsic blood coagulation; During coagulation, factor IX is converted to an enzyme,factor IX;l, by factor XIa- Factor IXa then converts factor X (Stuart factor) to factor Xa in the presence of factor VIII. Factor IX is a factor of the blood coagulation system. Its activation occurs on the surface of phospholipid membranes. |
| Biochemical Properties | single polypeptide chain with an amino-terminal sequence of Tyr-Asn-Ser-Gly; isoelectric point is 3.7; binds to Dextran Blue in the presence of calcium ions; susceptible to minor protease degradation by plasma enzymes |
| PTMs | Glycosylated: contains approximately 26% carbohydrate which includes 10.6% hexose, 6.5% V-acetylhexosamine, and 8.7% V-acetyIncuraminic acid; |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P00741|FA9_BOVIN Coagulation factor IX OS=Bos taurus OX=9913 GN=F9 PE=1 SV=2 MWCLNMIMAESPGLVTICLLGYLLSAECTVFLDRENATKILHRPKRYNSGKLEEFVRGNLERECKEEKCSFEEAREVFENTEKTTEFWKQYVDGDQCES*99NPCLNGGMCKDDINSYECWCQAGFEGTNCELDATCSIKNGRCKQFCKRDTDNKVVCSCTDGYRLAEDQKSCEPAVPFPCGRVSVSHISKKLTRAETIFSNTNYEN*204SSEAEIIWDN*214VTQSN*219QSFDEFSRVVGGEDAERGQFPWQVLLHGEIAAFCGGSIVNEKWVVTAAHCIKPGVKITVVAGEHNTEKPEPTEQKRNVIRAIPYHSYN*307ASINKYSHDIALLELDEPLELNSYVTPICIADRDYTNIFLKFGYGYVSGWGKVFNRGRSASILQYLKVPLVDRATCLRSTKFIYSHMFCAGYHEGGKDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT |
| SCOP | Class : Small proteins;Proteins with little or no secondary structures. Fold : GLA-domain Superfamily : GLA-domain Family : GLA-domain Domain Name : 1J34 C:401-446 |
| CATH | Matched CATH superfamily 3.10.100.10 |
| Predicted Disorder Regions | NA |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| Significance of PTMs | high carbohydrate content of factor IX, a carbohydrate heparin interaction bridged by calcium ions is possible |
| PDB ID | 1J34, 1J35, |
| Bibliography | 1. Fujikawa, K. et al. (1973) ‘Isolation and characterization of bovine factor IX (Christmas factor)’, Biochemistry, 12(24), pp. 4938–4945. doi: 10.1021/bi00748a019. 2. Fujikawa, K. et al. (1974) ‘Mechanism of activation of bovine factor IX (Christmas factor) by bovine factor XIa(activated plasma thromboplastin antecedent)’, Biochemistry, 13(22), pp. 4508–4516. doi: 10.1021/bi00719a006. 2.Taran LD. Factor IX of the blood coagulation system: a review. Biochemistry (Mosc). 1997 Jul;62(7):685-93. PMID: 9331959. |