Primary Information |
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| BoMiProt ID | Bomi284 |
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| Protein Name | Deoxyribonuclease (Fragment) |
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| Organism | Bos taurus |
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| Uniprot ID | F1MW13 |
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| Milk Fraction | MFGM, Exosome |
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| Aminoacid Length | 316 |
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| Molecular Weight | 35174 |
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| FASTA Sequence |
Download |
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| Gene Name | DNASE1L1 |
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| Protein Existence Status | Unreviewed: Protein inferred from homology |
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Secondary Information |
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| Presence in other biological fluids/tissue/cells | Pancreas, Kidney ,Liver ,Spleen ,Heart ,Lung ,Stomach ,Small intestine,Cerebrum ,Cerebellum
,Parotid gland ,Submaxillary gland ,Testis ,Epididymis |
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| Protein Function | perform a variety of
important cellular roles by degrading DNA via hydrolysis of its phosphodiester backbone; cleave single or double-stranded DNA
and require divalent metal ions to hydrolyze DNA yielding 3
-hydroxyl and 5
-phosphorylated products |
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| Biochemical Properties | divalent cation-dependent endonuclease originally isolated from bovine pancreas; G-actin is known to be a potent
inhibitor of mammalian DNase I -the activity of mouse DNase
I was inhibited as readily as that of human DNase I by G-actin; all DNase
I family proteins contain hydrophobic precursor peptides in their
N-termini, two essential histidine residues of DNase I are
conserved in the other members, all are activated by Ca2+
and Mg2+ and are strongly inhibited by
Zn2+, and members hydrolyse DNA endonucleolytically to
produce 3' -OH/5' -P ends; DNase X has an
extra hydrophobic stretch in its C-terminus; optimal activity
at the low pH environment of lysosomes |
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| Significance in milk | protect
against viruses in milk owing to the ability to inhibit the activity of RNAdependent DNA-polymerase |
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| PTMs | Glycosylated: In humans, N-linked glycosylation at Asn-243- conserved in the corresponding
regions of monkey, porcine and bovine DNase X proteins |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Bibliography | 1. Takeshita, H. et al. (1997) ‘Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution.’, Biochemistry and molecular biology international, 42(1), pp. 65–75. Available at: http://www.ncbi.nlm.nih.gov/pubmed/9192086 (Accessed: 4 October 2019).
2. Shiokawa, D. et al. (2005) ‘Physical and biochemical properties of mammalian DNase X proteins: non-AUG translation initiation of porcine and bovine mRNAs for DNase X’, Biochemical Journal, 392(3), pp. 511–517. doi: 10.1042/BJ20051114.
3. Varela-Ramirez, A. et al. (2017) ‘Structure of acid deoxyribonuclease’, Nucleic Acids Research, 45(10), pp. 6217–6227. doi: 10.1093/nar/gkx222. |
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