Search by BoMiProt ID - Bomi2834

Primary Information

BoMiProt ID Bomi2834
Protein Name General vesicular transport factor p115
Organism Bos taurus
Uniprot IDP41541
Milk FractionExosome
Ref Sequence ID NP_777270.2
Aminoacid Length 961
Molecular Weight 107515
FASTA Sequence Download
Gene Name USO1
Gene ID 317724
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Protein Function Uso1 (yeast homolog of human p115) is essential for tethering in vesicle transport from ER to Golgi and interacts with Ypt1 GTPase.
Biochemical Properties p115 is an elongated homodimer with two globular "heads" and an extended "tail" reminiscent of myosin II.The predicted 108-kDa bovine protein consists of an N-terminal 73-kDa globular domain followed by a 29-kDa coiled-coil dimerization domain, a linker segment of 4 kDa, and a highly acidic domain of 3 kDa. p115 is related to Uso1p, a protein required for endoplasmic reticulum to Golgi vesicular transport in Saccharomyces cerevisiae, which has a similar "head-coil-acid" domain structure. The p115 and Uso1p heads are similar in size, have approximately 25% sequence identity, and possess two highly homologous regions (62% and 60% identity over 34 and 53 residues, respectively). There is a third region of homology (50% identity over 28 residues) between the coiled-coil and acidic domains.
PTMs Phosphorylated on Ser-942,N6-acetylation at Lysine
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 13-19,97-104,955-961
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs P115 is phosphorylated on Ser-942, and this modification appears to control p115 association with membranes.
Bibliography 1.Brandon E, Gao Y, Garcia-Mata R, Alvarez C, Sztul E. Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic. Eur J Cell Biol. 2003 Aug;82(8):411-20. doi: 10.1078/0171-9335-00327. PMID: 14533739. 2.Sapperstein SK, Walter DM, Grosvenor AR, Heuser JE, Waters MG. p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p. Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):522-6. doi: 10.1073/pnas.92.2.522. PMID: 7831323; PMCID: PMC42773. 3.Heo Y, Yoon HJ, Ko H, Jang S, Lee HH. Crystal structures of Uso1 membrane tether reveal an alternative conformation in the globular head domain. Sci Rep. 2020 Jun 12;10(1):9544. doi: 10.1038/s41598-020-66480-1. PMID: 32533038; PMCID: PMC7293329.