Primary Information |
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| BoMiProt ID | Bomi2791 |
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| Protein Name | Prothrombin |
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| Organism | Bos taurus |
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| Uniprot ID | P00735 |
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| Milk Fraction | Whey, Exosome |
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| Ref Sequence ID | NP_776302.1 |
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| Aminoacid Length | 625 |
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| Molecular Weight | 70506 |
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| FASTA Sequence |
Download |
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| Gene Name | F2 |
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| Gene ID | 280685 |
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| Protein Existence Status | Reviewed: Experimental evidence at protein level |
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Secondary Information |
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| Protein Function | Prothrombin plays a key role in hemostasis also suggests that it may be important for mouse development. |
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| Biochemical Properties | In general, a high degree of homology in the primary structure of prothrombin and intermediates was observed between the human and bovine system. The NH2-terminal sequences of human intermediate 2' and alpha-thrombin A chain are identical. However, human intermediate 2' isolated in a manner identical with that used for the isolation of bovine intermediate 2 is homologous with bovine intermediate 2, beginning with residue 14. |
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| PTMs | Cleavage on pair of basic residues, Disulfide bond formation, N-Linked Glycosylation at Asn, Zymogen formation,Attachment of Gamma-carboxyglutamic acid |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. |
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| Additional Comments | Prothrombin deficiency in mice results in partial embryonic lethality. |
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| Bibliography | 1.Degen SJ, Sun WY. The biology of prothrombin. Crit Rev Eukaryot Gene Expr. 1998;8(2):203-24. doi: 10.1615/critreveukargeneexpr.v8.i2.60. PMID: 9714898. 2.Magnusson S., Sottrup-Jensen L., Petersen T.E., Claeys H.(In) Hemker H.C., Veltkamp J.J. (eds.); Boerhaave symposium on prothrombin and related coagulation factors, pp.25-46, Leiden University Press, Leiden (1975) |
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