Primary Information |
---|
BoMiProt ID | Bomi2791 |
---|
Protein Name | Prothrombin |
---|
Organism | Bos taurus |
---|
Uniprot ID | P00735 |
---|
Milk Fraction | Whey, Exosome |
---|
Ref Sequence ID | NP_776302.1 |
---|
Aminoacid Length | 625 |
---|
Molecular Weight | 70506 |
---|
FASTA Sequence |
Download |
---|
Gene Name | F2 |
---|
Gene ID | 280685 |
---|
Protein Existence Status | Reviewed: Experimental evidence at protein level |
---|
Secondary Information |
---|
Protein Function | Prothrombin plays a key role in hemostasis also suggests that it may be important for mouse development. |
---|
Biochemical Properties | In general, a high degree of homology in the primary structure of prothrombin and intermediates was observed between the human and bovine system. The NH2-terminal sequences of human intermediate 2' and alpha-thrombin A chain are identical. However, human intermediate 2' isolated in a manner identical with that used for the isolation of bovine intermediate 2 is homologous with bovine intermediate 2, beginning with residue 14. |
---|
PTMs | Cleavage on pair of basic residues, Disulfide bond formation, N-Linked Glycosylation at Asn, Zymogen formation,Attachment of Gamma-carboxyglutamic acid |
---|
Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| |
---|
Predicted Disorder Regions | NA |
---|
DisProt Annotation | |
---|
TM Helix Prediction | No TM helices |
---|
Significance of PTMs | The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. |
---|
Additional Comments | Prothrombin deficiency in mice results in partial embryonic lethality. |
---|
Bibliography | 1.Degen SJ, Sun WY. The biology of prothrombin. Crit Rev Eukaryot Gene Expr. 1998;8(2):203-24. doi: 10.1615/critreveukargeneexpr.v8.i2.60. PMID: 9714898. 2.Magnusson S., Sottrup-Jensen L., Petersen T.E., Claeys H.(In) Hemker H.C., Veltkamp J.J. (eds.); Boerhaave symposium on prothrombin and related coagulation factors, pp.25-46, Leiden University Press, Leiden (1975) |