|Ref Sequence ID||NP_001095313.1|
|Protein Existence Status||Reviewed: Experimental evidence at transcript level|
|Presence in other biological fluids/tissue/cells||placenta, lung, heart, astrocytes, microglia, vascular/perivascular cells of the brain and in cultured nurse-like cell lines|
|Protein Function||mediate phagocytosis of Saccharomyces,E. coli and S. aureus; Cl-P1 in the vascular spaces acts as a scavenger receptor to scavenge released neutrophil glycoproteins, both locally at sites of inflammation or systemically,when they are released in the circulation; CL-P1 bound CRP in a charge-dependent manner interaction mediated activation of the classical complement activation pathway. Collectin-12 (collectin placenta 1, CL-P1, or CL-12) is a newly identified pattern recognition molecule of the innate immune system. Recent evidences show that CL-12 plays important roles not only in innate immune protection against certain clinically important pathogens but also in scavenging of host molecules, leukocyte recruitment, and cancer metastasis.|
|Biochemical Properties||single pass (type II) transmembrane protein orientated with its N-terminal towards the cytosol; trimer of polypeptide chains, initiated and stabilized by formation of the α helical coiled-coil and the collagen-like region; regarded as both a collectin and a scavenger receptor; possesses three positive charged clusters in the collagen-like region, which are highly conserved in CL-P1 from various species; does not bind to the majority of Lewis structures that are sialylated|
|Significance in milk||Antimicrobial proteins - exclusively detected in the colostrum fluid phase|
|PTMs||Disulfide bond formation and N-Linked Glycosylation at Asn|
| Site(s) of PTM(s) |
|Predicted Disorder Regions||439-591|
|TM Helix Prediction||1TMH; (40-62)|
|Bibliography||1. Ohtani, K. et al. (2001) ‘The Membrane-type Collectin CL-P1 Is a Scavenger Receptor on Vascular Endothelial Cells’, Journal of Biological Chemistry, 276(47), pp. 44222–44228. doi: 10.1074/jbc.M103942200. |
2. Nakamura, K. et al. (2001) ‘Molecular Cloning and Functional Characterization of a Human Scavenger Receptor with C-Type Lectin (SRCL), a Novel Member of a Scavenger Receptor Family’, Biochemical and Biophysical Research Communications, 280(4), pp. 1028–1035. doi: 10.1006/bbrc.2000.4210.
3. Mori, K. et al. (2014) ‘Scavenger receptor CL-P1 mainly utilizes a collagen-like domain to uptake microbes and modified LDL’, Biochimica et Biophysica Acta (BBA) - General Subjects, 1840(12), pp. 3345–3356. doi: 10.1016/j.bbagen.2014.08.015.
4. Yoshida, T. et al. (2003) ‘SRCL/CL-P1 recognizes GalNAc and a carcinoma-associated antigen, Tn antigen.’, Journal of biochemistry, 133(3), pp. 271–7. doi: 10.1093/jb/mvg037.
5. Roy, N. et al. (2016) ‘Collectin CL-P1 utilizes C-reactive protein for complement activation’, Biochimica et Biophysica Acta (BBA) - General Subjects, 1860(6), pp. 1118–1128. doi: 10.1016/j.bbagen.2016.02.012.
6. Jang, S. et al. (2009) ‘Scavenger Receptor Collectin Placenta 1 (CL-P1) Predominantly Mediates Zymosan Phagocytosis by Human Vascular Endothelial Cells’, Journal of Biological Chemistry, 284(6), pp. 3956–3965. doi: 10.1074/jbc.M807477200.
7. Ohtani, K. et al. (1999) ‘Molecular Cloning of a Novel Human Collectin from Liver (CL-L1)’, Journal of Biological Chemistry, 274(19), pp. 13681–13689. doi: 10.1074/jbc.274.19.13681. 8.Zhang J, Li A, Yang CQ, Garred P, Ma YJ. Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens. J Immunol Res. 2019 Jul 29;2019:9164202. doi: 10.1155/2019/9164202. PMID: 31482100; PMCID: PMC6701420.