Search by BoMiProt ID - Bomi2292

Primary Information

BoMiProt ID Bomi2292
Protein Name Guanine nucleotide-binding protein G(i) subunit alpha-1
Organism Bos taurus
Uniprot IDP63097
Milk FractionMFGM, Exosome
Ref Sequence ID NP_776749.1
Aminoacid Length 354
Molecular Weight 40361
FASTA Sequence Download
Gene Name GNAI1
Gene ID 281790
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Protein Function The alpha subunits of the stimulatory and inhibitory G proteins, Gs alpha and Gi alpha, activate transmembrane-signaling systems involved in the control of cell proliferation. 
Biochemical Properties A much higher degree of amino acid sequence homology is observed between the alpha-subunits of Gi and transducin (68%) than between those of Gi and the adenylate cyclase-stimulating G-protein (Gs) (43%) or between those of transducin and Gs (42%).
PTMs Myristoylation at Gly-2,Palmitoylation at Cys-3
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions 5-32,203-214,343-354
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Myristoylation at Gly-2 takes place for membrane anchoring before palmitoylation.
PDB ID 6K42, 6NBF, 6NBH, 6NBI, 7D68,
Additional Comments The constitutive expression of Gi alpha-1 in autonomous adenoma may allow for the unregulated stimulation of thyroid cell proliferation.
Bibliography 1.Nukada T, Tanabe T, Takahashi H, Noda M, Haga K, Haga T, Ichiyama A, Kanagawa K, Hiranaga M, Matsuo, et al. Primary structure of the alpha-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence. FEBS Lett. 1986 Mar 3;197(1-2):305-10. doi: 10.1016/0014-5793(86)80347-7. PMID: 2419165. 2.Selzer E, Wilfing A, Schiferer A, Hermann M, Grubeck-Loebenstein B, Freissmuth M. Stimulation of human thyroid growth via the inhibitory guanine nucleotide binding (G) protein Gi: constitutive expression of the G-protein alpha subunit Gi alpha-1 in autonomous adenoma. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1609-13. doi: 10.1073/pnas.90.4.1609. PMID: 8434024; PMCID: PMC45924.