Search by BoMiProt ID - Bomi2200

Primary Information

BoMiProt ID Bomi2200
Protein Name Glyceraldehyde-3-phosphate dehydrogenase
Organism Bos taurus
Uniprot IDP10096
Milk FractionWhey, MFGM, Exosome
Ref Sequence ID NP_001029206.1
Aminoacid Length 333
Molecular Weight 35868
FASTA Sequence Download
Gene Name GAPDH
Gene ID 281181
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Protein Function Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a moonlighting protein exhibiting distinct activities apart from its classical role in glycolysis.
PTMs N6-Acetylation at Lys, ADP-ribosylation, Isopeptide bond formation,N6,N6-dimethylation at Lysine, Phosphorylation at Ser/Thr, S-nitrosylation, Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus S-nitrosylation of Cys-245 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.Sulfhydration at Cys-150 increases catalytic activity.
PDB ID 4O59, 4O63,
Bibliography Sirover MA. The role of posttranslational modification in moonlighting glyceraldehyde-3-phosphate dehydrogenase structure and function. Amino Acids. 2021 Apr;53(4):507-515. doi: 10.1007/s00726-021-02959-z. Epub 2021 Mar 2. PMID: 33651246.