Primary Information | |
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BoMiProt ID | Bomi2200 |
Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
Organism | Bos taurus |
Uniprot ID | P10096 |
Milk Fraction | Whey, MFGM, Exosome |
Ref Sequence ID | NP_001029206.1 |
Aminoacid Length | 333 |
Molecular Weight | 35868 |
FASTA Sequence | Download |
Gene Name | GAPDH |
Gene ID | 281181 |
Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
Protein Function | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a moonlighting protein exhibiting distinct activities apart from its classical role in glycolysis. |
PTMs | N6-Acetylation at Lys, ADP-ribosylation, Isopeptide bond formation,N6,N6-dimethylation at Lysine, Phosphorylation at Ser/Thr, S-nitrosylation, Ubl conjugation |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | |
Predicted Disorder Regions | NA |
DisProt Annotation | |
TM Helix Prediction | No TM helices |
Significance of PTMs | S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus S-nitrosylation of Cys-245 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.Sulfhydration at Cys-150 increases catalytic activity. |
PDB ID | 4O59, 4O63, |
Bibliography | Sirover MA. The role of posttranslational modification in moonlighting glyceraldehyde-3-phosphate dehydrogenase structure and function. Amino Acids. 2021 Apr;53(4):507-515. doi: 10.1007/s00726-021-02959-z. Epub 2021 Mar 2. PMID: 33651246. |