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Primary Information | |
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| BoMiProt ID | Bomi214 |
| Protein Name | Cytosol aminopeptidase |
| Organism | Bos taurus |
| Uniprot ID | P00727 |
| Milk Fraction | Exosome |
| Ref Sequence ID | NP_776523.2 |
| Aminoacid Length | 519 |
| Molecular Weight | 56289 |
| FASTA Sequence | Download |
| Gene Name | LAP3 |
| Gene ID | 781648 |
| Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
| Protein Function | catalyze the rapid hydrolysis of aminoacyl-/3-naphthylamides. Leucine aminopeptidase (LAP, EC: 3.4.11.1) is an important metalloexopeptidase that catalyze the hydrolysis of amino-terminal leucine residues from polypeptides and proteins. |
| Biochemical Properties | broader specificity while hydrolyzing Leu substrates; have variable temperature and pH optima and divalent cation requirements; |
| Significance in milk | show high potential in removing the bitterness of milk by hydrolysis of polypeptides |
| PTMs | N6-acetyllysine,Phosphorylation at Serine |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | |
| SCOP | Class : Alpha and beta proteins (a/b) Fold : Phosphorylase/hydrolase-like Superfamily : Zn-dependent exopeptidases Family : Leucine aminopeptidase, C-terminal domain Domain Name : 1LAM A:160-484 Class : Alpha and beta proteins Fold : Macro domain-like Superfamily : Macro domain-like Family : PepA N-terminal domain-like Domain Name : 1LAM A:1-159 |
| CATH | Matched CATH superfamily 3.40.630.10 3.40.220.10 |
| Predicted Disorder Regions | 48-51 |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| PDB ID | 1BLL, 1BPM, 1BPN, 1LAM, 1LAN, 1LAP, 1LCP, 2EWB, 2J9A, |
| Bibliography | 1. TALANTI, S., & HOPSU, V. K. (1961). Leucine aminopeptidase in the mammary gland of the cow and rat. Nature, 191, 86–87. https://doi.org/10.1038/191086b0. 2. Jóźwik, A., Bagnicka, E., Śliwa-Jóźwik, A., Strzałkowska, N., Sloniewski, K., Krzyżewski, J., & Kolataj, A. (2004). Activity of selected aminopeptidases of whole milk in cows as related to feeding season (autumn/winter vs spring/summer).. 3. Starnes, W. L., & Behal, F. J. (1974). A human liver aminopeptidase. The amino acid and carbohydrate content, and some physical properties of a sialic acid containing glycoprotein. Biochemistry, 13(16), 3221–3227. https://doi.org/10.1021/bi00713a004. 4.Zhang S, Cai X, Luo X, Wang S, Guo A, Hou J, Wu R. Molecular cloning and characterization of leucine aminopeptidase gene from Taenia pisiformis. Exp Parasitol. 2018 Mar;186:1-9. doi: 10.1016/j.exppara.2018.01.012. Epub 2018 Jan 10. PMID: 29329981. |