Search by BoMiProt ID - Bomi214


Primary Information

BoMiProt ID Bomi214
Protein Name Cytosol aminopeptidase
Organism Bos taurus
Uniprot IDP00727
Milk FractionExosome
Ref Sequence ID NP_776523.2
Aminoacid Length 519
Molecular Weight 56289
FASTA Sequence Download
Gene Name LAP3
Gene ID 781648
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Protein Function catalyze the rapid hydrolysis of aminoacyl-/3-naphthylamides. Leucine aminopeptidase (LAP, EC: 3.4.11.1) is an important metalloexopeptidase that catalyze the hydrolysis of amino-terminal leucine residues from polypeptides and proteins.
Biochemical Properties broader specificity while hydrolyzing Leu substrates; have variable temperature and pH optima and divalent cation requirements;
Significance in milk show high potential in removing the bitterness of milk by hydrolysis of polypeptides
PTMs N6-acetyllysine,Phosphorylation at Serine
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
SCOP Class : Alpha and beta proteins (a/b)
Fold : Phosphorylase/hydrolase-like
Superfamily : Zn-dependent exopeptidases
Family : Leucine aminopeptidase, C-terminal domain
Domain Name : 1LAM A:160-484

Class : Alpha and beta proteins
Fold : Macro domain-like
Superfamily : Macro domain-like
Family : PepA N-terminal domain-like
Domain Name : 1LAM A:1-159

CATH Matched CATH superfamily
3.40.630.10
3.40.220.10
Predicted Disorder Regions 48-51
DisProt Annotation
TM Helix Prediction No TM helices
PDB ID 1BLL, 1BPM, 1BPN, 1LAM, 1LAN, 1LAP, 1LCP, 2EWB, 2J9A,
Bibliography 1. TALANTI, S., & HOPSU, V. K. (1961). Leucine aminopeptidase in the mammary gland of the cow and rat. Nature, 191, 86–87. https://doi.org/10.1038/191086b0.
2. Jóźwik, A., Bagnicka, E., Śliwa-Jóźwik, A., Strzałkowska, N., Sloniewski, K., Krzyżewski, J., & Kolataj, A. (2004). Activity of selected aminopeptidases of whole milk in cows as related to feeding season (autumn/winter vs spring/summer)..
3. Starnes, W. L., & Behal, F. J. (1974). A human liver aminopeptidase. The amino acid and carbohydrate content, and some physical properties of a sialic acid containing glycoprotein. Biochemistry, 13(16), 3221–3227. https://doi.org/10.1021/bi00713a004. 4.Zhang S, Cai X, Luo X, Wang S, Guo A, Hou J, Wu R. Molecular cloning and characterization of leucine aminopeptidase gene from Taenia pisiformis. Exp Parasitol. 2018 Mar;186:1-9. doi: 10.1016/j.exppara.2018.01.012. Epub 2018 Jan 10. PMID: 29329981.