Search by BoMiProt ID - Bomi207

Primary Information

BoMiProt ID Bomi207
Protein Name Acyl-CoA-binding protein
Organism Bos taurus
Uniprot IDP07107
Milk FractionExosome
Ref Sequence ID NP_001106792.1
Aminoacid Length 87
Molecular Weight 10044
FASTA Sequence Download
Gene Name DBI
Gene ID 768330
Protein Existence Status Reviewed: Experimental evidence at protein level

Secondary Information

Presence in other biological fluids/tissue/cells mainly cytosolic, and the highest concentration is found in liver
Protein Function potential role in acyl-CoA metabolism, the protein may have neurotransmitter activity; important role in intracellular acyl-CoA transport and pool formation and therefore also for the function of long chain acyl coA esters as metabolites and regulators of cellular functions; play a role in the sequestration, transport, and distribution of longchain acyl-CoAs in cells. Neural stem cell pro-proliferative factor acyl-CoA-binding protein (ACBP, also known as DBI) is highly expressed in GBM, and by binding to acyl-CoAs, it cell-autonomously maintains high proliferation rates, promoting tumor growth and poor survival in several preclinical models.
Biochemical Properties Bovine and rat liver acyl-CoA-binding proteins (ACBP) were found to exhibit a much higher affinity for long-chain acyl-CoA esters than both bovine hepatic and cardiac fatty-acid-binding proteins; bind acyl- CoA esters, but not fatty acids; bind saturated acyl-CoA esters with chain lengths from 8 to 18 carbon atoms with high affinity, but is unable to bind free fatty acids; FABP from bovine heart and liver exhibited much lower affinity for both [1-4C]hexadecanoyl-CoA and cis- 9-[1-14C]octadecenoyl-CoA than bovine and rat liver ACBP; bovine liver (l-ACBP) contains no cysteine; t-ACBP have now been isolated from three different species and all testes specific-ACBP contain three cysteine; The binding site is located in a hydrophobic groove on the surface of liver-ACBP; l-ACBP does not bind fatty acids, acyl carnitines, cholesterol and a number of nucleotides; the binding affinities decrease with increasing ionic strength of the buffer
Significance in milk involved in acetate and FA activation and intracellular transport in mammary gland
PTMs N6-acetylation at Lysine,Phosphorylation at Tyrosine
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
SCOP Class : All alpha proteins
Fold : Acyl-CoA binding protein-like
Superfamily : Acyl-CoA binding protein
Family : Acyl-CoA binding protein
Domain Name : 1HB6 A:1-86

CATH Matched CATH superfamily
Predicted Disorder Regions 1-11,14-24,82-87
DisProt Annotation
TM Helix Prediction No TM helices
PDB ID 1ACA, 1HB6, 1HB8, 1NTI, 1NVL, 2ABD,
Additional Comments Acyl-CoA-binding protein (ACBP) drives glioblastoma growth by promoting mitochondrial long fatty acyl-CoA accumulation and β-oxidation.
Bibliography 1. Rosendal, J., Ertbjerg, P., & Knudsen, J. (1993). Characterization of ligand binding to acyl-CoA-binding protein. The Biochemical Journal, 290 ( Pt 2), 321–326.
2. Shang, X., He, Y., Zhang, L., Chen, B., He, C. J., Cheng, H. H., & Zhou, R. J. (2006). Molecular cloning of the rice field Eel Nup93 with predominant expression in gonad and kidney. Acta Genetica Sinica, 33(1), 41–48.
3. Soupene, E., Serikov, V., & Kuypers, F. A. (2008). Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells. Journal of Lipid Research, 49(5), 1103–1112.
4. Bionaz, M., & Loor, J. J. (2008). Gene networks driving bovine milk fat synthesis.pdf. BioMed Central, 9, 366.
5. Rasmussen, J. T., Börchers, T., & Knudsen, J. (1990). Comparison of the binding affinities of acyl-CoA-binding protein and fatty-acid-binding protein for long-chain acyl-CoA esters. The Biochemical Journal, 265(3), 849–855.
6. Whetstone, H. D., Hurley, W. L., & Davis, C. L. (1986). Identification and characterization of a fatty acid binding protein in bovine mammary gland. Comparative Biochemistry and Physiology. B, Comparative Biochemistry, 85(3), 687–692.
7. Mikkelsen, J., & Knudsen, J. (1987). Acyl-CoA-binding protein from cow. Binding characteristics and cellular and tissue distribution. The Biochemical Journal, 248(3), 709–714. 8.Duman C, Yaqubi K, Hoffmann A, Acikgöz AA, Korshunov A, Bendszus M, Herold-Mende C, Liu HK, Alfonso J. Acyl-CoA-Binding Protein Drives Glioblastoma Tumorigenesis by Sustaining Fatty Acid Oxidation. Cell Metab. 2019 Aug 6;30(2):274-289.e5. doi: 10.1016/j.cmet.2019.04.004. Epub 2019 May 2. PMID: 31056285. 9.Bi J, Mischel PS. Acyl-CoA-Binding Protein Fuels Gliomagenesis. Cell Metab. 2019 Aug 6;30(2):229-230. doi: 10.1016/j.cmet.2019.07.007. PMID: 31390546.