Primary Information
BoMiProt ID	Bomi160
Protein Name	Collectin-46
Organism	Bos taurus
Uniprot ID	Q8MHZ9
Milk Fraction	Whey
Ref Sequence ID	NP_001001856.1
Aminoacid Length	371
Molecular Weight	37445
FASTA Sequence	Download
Gene Name	CL46
Gene ID	415114
Protein Existence Status	Reviewed: Experimental evidence at transcript level
Secondary Information
Presence in other biological fluids/tissue/cells	dominant place of CL-46 expression is the thymus; also present in liver and moderate expression was detected in the mammary gland and in tissues throughout the digestive system
Protein Function	involved in the innate immune defense; bind to microbial surface carbohydrates inducing aggregation and, thereby, impeding infectivity; binds to the collectin receptor (C1qR) localized on many types of cells identified as a surface variant of calreticulin; show antiviral activities towards influenza A virus and rotaviruses ; displays protective activity against bacterial infections
Biochemical Properties	form tetramers; stabilized by noncovalent interactions (strong hydrophobic forces in α-helical bundle) and covalently by disulfide bridges between cysteine residues in the N-terminal or collagen regions; high resemblance with conglutinin; CL-46 bind to a range of sugar residues including mannose, fucose, glucose, maltose, GlcNAc or ManNAc
Significance in milk	provide the first line of defence against these microbes
PTMs	Potential N-glycosylation site is localized at asparagine 90 in the collagen-like region
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation
Predicted Disorder Regions	43-215
DisProt Annotation
TM Helix Prediction	No TM helices
Bibliography	1. Nissen, A., Andersen, P. H., Bendixen, E., Ingvartsen, K. L., and Røntved, C. M. (2017) Colostrum and milk protein rankings and ratios of importance to neonatal calf health using a proteomics approach. J. Dairy Sci. 100, 2711–2728. 2. Lu, J. (1997) Collectins: Collectors of microorganisms for the innate immune system. BioEssays 19, 509–518. 3. Hansen, S., Holm, D., Moeller, V., Vitved, L., Bendixen, C., Reid, K. B. M., Skjoedt, K., and Holmskov, U. (2002) CL-46, a novel collectin highly expressed in bovine thymus and liver. J. Immunol. 169, 5726–5734. 4. Holmskov, U., Laursen, S. B., Malhotra, R., Wiedemann, H., Timpl, R., Stuart, G. R., Tornøe, I., Madsen, P. S., Reid, K. B., and Jensenius, J. C. (1995) Comparative study of the structural and functional properties of a bovine plasma C-type lectin, collectin-43, with other collectins. Biochem. J. 305 ( Pt 3), 889–896. 5. Lim, B. L., Willis, A. C., Reid, K. B., Lu, J., Laursen, S. B., Jensenius, J. C., and Holmskov, U. (1994) Primary structure of bovine collectin-43 (CL-43). Comparison with conglutinin and lung surfactant protein-D. J. Biol. Chem. 269, 11820–11824. 6. Haurum, J. S., Thiel, S., Haagsman, H. P., Laursen, S. B., Larsen, B., and Jensenius, J. C. (1993) Studies on the carbohydrate-binding characteristics of human pulmonary surfactant-associated protein A and comparison with two other collectins: mannan-binding protein and conglutinin. Biochem. J. 293 ( Pt 3), 873–878. 7. Holmskov, U., Teisner, B., Willis, A. C., Reid, K. B., and Jensenius, J. C. (1993) Purification and characterization of a bovine serum lectin (CL-43) with structural homology to conglutinin and SP-D and carbohydrate specificity similar to mannan-binding protein. J. Biol. Chem. 268, 10120–10125. 8. Loveless, R. W., Holmskov, U., and Feizi, T. (1995) Collectin-43 is a serum lectin with a distinct pattern of carbohydrate recognition. Immunology 85, 651–659.