Primary Information
BoMiProt ID	Bomi150
Protein Name	Alpha-fetoprotein
Organism	Bos taurus
Uniprot ID	Q3SZ57
Milk Fraction	Whey
Ref Sequence ID	NP_001029434.1
Aminoacid Length	610
Molecular Weight	68588
FASTA Sequence	Download
Gene Name	AFP
Gene ID	506011
Protein Existence Status	Reviewed: Experimental evidence at transcript level
Secondary Information
Presence in other biological fluids/tissue/cells	fetal liver, yolk sac of mammals; bovine fetal fluids; ascitic fluid, cerebrospinal fluid (CSF) and bile, from human fetuses;
Protein Function	contribute to the efficiency of stimulation of proliferation in cell culture systems; growth-promoting activity;
Biochemical Properties	charge microheterogeneity in the bAFP indirectly suggests that the multiple bands may be due to carbohydrate; charge profile of bovine AFPsignificantly more alkaline than that observed in the human; greater degree of charge microheterogeneity than the human;
Significance in milk	Innate immunity agents in milk
PTMs	glycoprotein; bAFP in FBS is approximately evenly divided between Con A reactive and non-reactive variants; bAFP in amniotic fluid from an early gestation animal exhibited 72% reactive and 28% non-reactive variants
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation
Predicted Disorder Regions	NA
DisProt Annotation
TM Helix Prediction	No TM helices
Significance of PTMs	significant changes in lectin-binding properties of glycoproteins are associated with normal and tumor cell growth as well as fetal development; non-reactive variants of bAFP in FBS increases in parallel with fetal development;
Bibliography	1. Smith, J. A., Francis, T. I., Edington, G. M., & Williams, A. O. (1971). Human alpha fetoprotein in body fluids. British Journal of Cancer, 25(2), 337–342. https://doi.org/10.1038/bjc.1971.43. 2. Janzen, R. G., Mably, E. R., Tamaoki, T., Church, R. B., & Lorscheider, F. L. (1982). Synthesis of alpha-fetoprotein by the pre-implantation and post-implantation bovine embryo. Journal of Reproduction and Fertility, 65(1), 105–110. https://doi.org/10.1530/jrf.0.0650105. 3. He, Y., & Keel, B. A. (1994). Biochemical characterization of bovine alpha-fetoprotein and comparison with human alpha-fetoprotein. Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology, 108(3), 327–336. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7521727.