Primary Information | |
---|---|
BoMiProt ID | Bomi116 |
Protein Name | Lactotransferrin |
Organism | Bos taurus |
Uniprot ID | P24627 |
Milk Fraction | Whey |
Ref Sequence ID | NP_851341.1 |
Aminoacid Length | 708 |
Molecular Weight | 78056 |
FASTA Sequence | Download |
Gene Name | LTF |
Gene ID | 280846 |
Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
Presence in other biological fluids/tissue/cells | saliva, tears, bile/pncreatic secretions, mucosal secretions of trachea, uterus, ovaries, seminal secretion, plasma, joint fluid |
Endogenous/Bioactive peptides - Fragment - Sequence - Effect | Lactoferricin - 17–41/42 - FKCRRWQWRMKKLGAPSICURRAF/A - Antimicrobial Ref |
Protein Function | Potent iron chelator; has antimicrobial, anti-tumor, anti-allergeic, anti-inflammatory activities; promotes innante and adaptive immunity and neutrophil migration; depresses endotoxemia; acts as tumor suppressor in multiple cancers; controls oxidative cell injury; inhibits pathological development during autoimmune disorder; prevents cell apoptosis; used as chemotherapeutic agent |
Biochemical Properties | Thermostable in acidic environment; optimum pH is 7.5 and temperature is 25°C with 20% α-helix structure; at pH 2-3 structure is partly unfolded with 3% α - helix structure and 54% ß - sheet structure; showed higher thermostability in acidic pH and low ionic strengths; relatively more resistant to heat in acid than in base or neutral solutions ; loses iron binding capacity but retains antimicrobial properties at extreme conditions such as pH 2, 120°C |
Significance in milk | Highest in colustrum; has biomodulatory properties and effects nutritional value of the milk; used as active ingredients in infant formula, prebiotic drinks and immune supplements; lactoferrin through pepsin digestion produces a more potent anti-microbial peptide, lactoferrisin that reaches the gut |
PTMs | Glycosylated; 5 N-linked glycans; mixtures of high mannose, complex and hybrid type; 59 different structures have been identified till date; fucosylated and sialylated; presence of unusual motifs including N,N'-diacetyllactosamine as found in bovine milk |
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P24627|TRFL_BOVIN Lactotransferrin OS=Bos taurus OX=9913 GN=LTF PE=1 SV=2 MKLFVPALLS LGALGLCLAA PRKNVRWCTI SQPEWFKCRR WQWRMKKLGA PSITCVRRAF ALECIRAIAE KKADAVTLDG GMVFEAGRDP YKLRPVAAEI YGTKESPQTH YYAVAVVKKG SNFQLDQLQG RKSCHTGLGR SAGWIIPMGI LRPYLSWTES LEPLQGAVAK FFSASCVPCI DRQAYPNLCQ LCKGEGENQC ACSSREPYFG YSGAFKCLQD GAGDVAFVKE TTVFENLPEK ADRDQYELLC LN*252NSRAPVDA FKECHLAQVP SHAVVARSVD GKEDLIWKLL SKAQEKFGKN KSRSFQLFGS PPGQRDLLFK DSALGFLRIP SKVDSALYLG SRYLTTLKNL RETAEEVKAR YTRVVWCAVG PEEQKKCQQW SQQSGQN*387VTC ATASTTDDCI VLVLKGEADA LNLDGGYIYT AGKCGLVPVL AENRKSSKHS SLDCVLRPTE GYLAVAVVKK ANEGLTWNSL KDKKSCHTAV DRTAGWNIPM GLIVN*495QTGSC AFDEFFSQSC APGADPKSRL CALCAGDDQG LDKCVPNSKE KYYGYTGAFR CLAEDVGDVA FVKN*564DTVWEN TNGESTADWA KNLNREDFRL LCLDGTRKPV TEAQSCHLAV APNHAVVSRS DRAAHVKQVL LHQQALFGKN GKNCPDKFCL FKSETKNLLF NDNTECLAKL GGRPTYEEYL GTEYVTAIAN LKKCSTSPLL EACAFLTR |
SCOP | Class : Alpha and beta proteins (a/b) Fold : SBP2HA-like Superfamily : Type 2 solute binding protein-like Family : Transferrin Domain Name : 3IB0 A:342-685 |
CATH | Matched CATH superfamily 3.40.190.10 |
Predicted Disorder Regions | NA |
DisProt Annotation | |
TM Helix Prediction | No TM helices |
Significance of PTMs | Glycosylation changes at multiple points of lactation; glycosylation plays a role in the protein's bioactivity and its digestion profile in the infant gut; source of novel bioactives; stimulation of bone formation; anticancer; sialylation chelates loosed bound Ca2+ and prevent bacterial colonization |
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