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Primary Information | |
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| BoMiProt ID | Bomi115 |
| Protein Name | Conglutinin |
| Organism | Bos taurus |
| Uniprot ID | P23805 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_783630.2 |
| Aminoacid Length | 371 |
| Molecular Weight | 37995 |
| FASTA Sequence | Download |
| Gene Name | CGN1 |
| Gene ID | 281068 |
| Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
| Protein Function | conglutinin in the serum of dairy cows depends on many factors such as breeding, the season of the year, the stage of the reproductive cycle and infection. Conglutinin acts as an opsonin for influenza A viruses. |
| Biochemical Properties | Conglutinin contain 4 trimeric units; there are 7 cysteine residues present which are capable of forming inter – and/or intramolecular disulfide bridges; The collagen- like region of conglutinin contains eight hydroxylysine residues, which are possible glycosylation sites; conglutinin molecules adhere to each other in the central lob region, forming rosette-like aggregates |
| Significance in milk | effector molecules in innate immunity; . Conglutinin does not appear in the serum of calves until after it has received colostrum; then its concentration gradually increases up to the 80th week of life, when it reaches the level characteristic for adult cattle |
| PTMs | presence of O-linked glycans of β(1-3)-GalNAc and α-(2-3) linked sialic acid type. No N-linked glycans were detected |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | >sp|P23805|CONG_BOVIN Conglutinin OS=Bos taurus OX=9913 GN=CGN1 PE=1 SV=2 MLLLPLSVLLLLTQPWRSLGAEMTTFSQKILANACTLVMCSPLESGLPGHDGQDGRECPH GEKGDPGSPGPAGRAGRPGWVGPIGPKGDNGFVGEPGPKGDTGPRGPPGMPGPAGREGPS GKQGSMGPPGTPGPKGETGPKGGVGAPGIQGFPGPSGLKGEKGAPGETGAPGRAGVTGPS GAIGPQGPSGARGPPGLKGDRGDPGETGAKGESGLAEVNALKQRVTILDGHLRRFQNAFS QYKKAVLFPDGQAVGEKIFKTAGAVKSYSDAEQLCREAKGQLASPRSSAENEAVTQMVRA QEKNAYLSMNDISTEGRFTYPTGEILVYSNWADGEPN*337NSDEGQPENCVEIFPDGKWNDVP CSKQLLVICEF |
| Predicted Disorder Regions | 18-30,44-206,333-339 |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| PDB ID | 6RYG, 6RYJ, 6RYM, 6RYN, |
| Additional Comments | Bovine conglutinin binds to an oligosaccharide determinant presented by iC3b, but not by C3, C3b or C3c. |
| Bibliography | 1. Dec, M., & Wernicki, A. (2006). Conglutinin, CL-43 and CL-46--three bovine collectins. Polish Journal of Veterinary Sciences, 9(4), 265–275. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/17203746. 2. Andersen, O., Nielsen, E. H., Storgaard, P., Højrup, P., Friis, P., Leslie, G., & Svehag, S. E. (n.d.). Biochemical and ultrastructural studies of the C-type lectin bovine conglutinin. Journal of Structural Biology, 109(3), 201–207. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1296754. 3.Laursen SB, Thiel S, Teisner B, Holmskov U, Wang Y, Sim RB, Jensenius JC. Bovine conglutinin binds to an oligosaccharide determinant presented by iC3b, but not by C3, C3b or C3c. Immunology. 1994 Apr;81(4):648-54. PMID: 7518801; PMCID: PMC1422359.4.Hartshorn KL, Sastry K, Brown D, White MR, Okarma TB, Lee YM, Tauber AI. Conglutinin acts as an opsonin for influenza A viruses. J Immunol. 1993 Dec 1;151(11):6265-73. PMID: 8245465. |