Primary Information |
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| BoMiProt ID | Bomi10792 |
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| Protein Name | Zinc finger SWIM domain-containing protein 8 |
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| Organism | Bos taurus |
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| Uniprot ID | A7E305 |
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| Milk Fraction | Whey,MFGM |
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| Ref Sequence ID | NP_001095619.1 |
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| Aminoacid Length | 1413 |
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| Molecular Weight | 152380 |
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| FASTA Sequence |
Download |
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| Gene Name | ZSWIM8 |
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| Gene ID | 533041 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Enables ubiquitin ligase-substrate adaptor activity. Involved in positive regulation of miRNA catabolic process; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein ubiquitination. |
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| Biochemical Properties | ZSWIM8 ubiquitin ligase recognizes conformational changes that occur upon extensive pairing to the miRNA 3-prime region, leading to polyubiquitination of AGO. Polyubiquitinated AGO is then degraded by the 26S proteasome, exposing the miRNA to cytoplasmic nucleases. contains a VHL box and SWIM domain and is thus recognized as a Cul2-type ubiquitin ligase. A SWIM domain is found in bacterial SWI2/SNF2 ATPases and MuDR transposases and is predicted to have DNA-binding and protein–protein interaction functions. |
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| Significance in milk | a myogenic protein |
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| PTMs | Phosphorylation at Ser/Thr |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A7E305|ZSWM8_BOVIN Zinc finger SWIM domain-containing protein 8 OS=Bos taurus OX=9913 GN=ZSWIM8 PE=2 SV=1
MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAES*36LCQNWRGWRKQS*48AGPNS*53PTGGGGG
GGSGGTRMRDGLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDI
RLYSCLANGSADEFQRGDQLFRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDR
CRVTSCSCTCGAGAKWCTHVVALCLFRIHNASAVCLRAPVSESLSRLQRDQLQKFAQYLI
SELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPSASDQSTWYLDESTLTDNIKK
TLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNLLSIVREMFKR
RDSNAAPLLEILTVELAQDLLANPPDLKVEPPPAKGKKNKVSTSRQTWVATNTLTKAAFL
LTVLSERPEHHNLAFRVGMFALELQRPPASTKALEVKLAYQESEVATLLKKIPLGPSEMS
TVRCRAEELREGTLCDYRPVLPLMLASFIFDVLCAPVVSPTGSRPPSRNWNNEMPGDEEL
GFEAAVAALGMKTTVSEAEHPLLCEGTRREKGDLALALMITYKDDQARLKKILDKLLDRE
SQTHKPQTLSSFYSSSRPATASQRSPSKHGGPSAPGALQPLTSGSAGPAQPGSVAGAGPG
PTEGFTEKNVPESSPHSPCEGLPPEAALTPRPEGKVPSRLALGSRGGYNGRGWGSPGRPK
KKHT*724GMASIDSSAPETTS*738DSS*741PTLS*745RRPLRGGWAPTSWGRGQDSDSISSSSSDSLGSSSSSGSRRASASGGARAKTVEVGRYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGN
SSTSIFTHPSSS*852GGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQA
MEIGSAALTILVECWDGHLTPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPN
EIQRALVQCKEQDNLMLEKACMAVEEAAKGGGVYPEVLFEVAHQWFWLYEQTAGGSSTAR
EGATSCSASGIRAAGEAGRGLPEGRGGPGTEPVTVAAAAVTAATVVPVISVGSSLYPGPG
LGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHMPRPAVFPVASSAYPQGVHPAF
LGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVALSSVHPASTFPAI
QGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLALEMLGRRAHN
DHPNNFSRSPPYTDDVKWLLGLAAKLGVNYVHQFCVGAAKGVLSPFVLQEIVMETLQRLS
PAHAHNHLRAPAFHQLVQRCQQAYMQYIHHRLIHLTPADYDDFVNAIRSARSAFCLTPMG
MMQFNDILQNLKRSKQTKELWQRVSLEMTTFSP
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| Predicted Disorder Regions | NA |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Additional Comments | Target-directed miRNA degradation (TDMD) required the ZSWIM8 Cullin-RING E3 ubiquitin ligase. |
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| Bibliography | 1.Shi CY, Kingston ER, Kleaveland B, Lin DH, Stubna MW, Bartel DP. The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation. Science. 2020 Dec 18;370(6523):eabc9359. doi: 10.1126/science.abc9359. Epub 2020 Nov 12. PMID: 33184237; PMCID: PMC8356967. 2.Okumura, F., Oki, N., Fujiki, Y. et al. ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation. Sci Rep 11, 20880 (2021). https://doi.org/10.1038/s41598-021-00306-6 |
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