Primary Information |
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| BoMiProt ID | Bomi10224 |
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| Protein Name | Ubiquitin carboxyl-terminal hydrolase BAP1/BRCA1-associated protein 1 |
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| Organism | Bos taurus |
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| Uniprot ID | A2VDM8 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001096019.1 |
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| Aminoacid Length | 711 |
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| Molecular Weight | 78333 |
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| FASTA Sequence |
Download |
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| Gene Name | BAP1 |
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| Gene ID | 100124510 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | a potent tumour suppressor gene ,localizes to ER,binds, deubiquitylates, and stabilizes type 3 inositol-1,4,5-trisphosphate receptor (IP3R3), modulating calcium (Ca2+) release from the endoplasmic reticulum into the cytosol and mitochondria, promoting apoptosis. |
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| Biochemical Properties | ubiquitin C-terminal hydrolase.Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
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| PTMs | ubl conjugation,Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A2VDM8|BAP1_BOVIN Ubiquitin carboxyl-terminal hydrolase BAP1 OS=Bos taurus OX=9913 GN=BAP1 PE=2 SV=1
MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRR
KVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSNVDLGPTLSRMKDFTK
GFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRL
FELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGIKYEARLHVLKVNRQTVLEA
LQQLIRVTQPELIQTHKSQESQLPEESKPASSKS*274PLALETSRAPVASESTHTDGVEEVAG
SCPQAPTHSPPSKPKLVVKPPGSNINGVPPNPTPIVQRLPAFLDNHNYAKS*351PMQEEEDLA
AGVGRSRVPVRPPQQYS*377DDEDDYEDEEEDDAQSTSSAIRYKRKGPGKPGPLSSSGDGQLS
VLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNEST*475DTASE
IGSAFNSPLRSPIRSANPTRPSS*503PVTSHISKVLFGEDDS*519LLRVDCIRYNRAVRDLGPVIS
TGLLHLAEDGVLSPLALTESGKGSSPS*567IRPSQGSQGSGS*579PEEKEVVEAVDSREKPGLVRP
SESLNGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTF
ISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ
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| Predicted Disorder Regions | 254-280, 294-342, 356-414, 427-512, 534-547, 560-589, 684-711 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | The ubiquitin-conjugating enzyme UBE2O induces BAP1 sequestration in the cytoplasm by multi-monoubiquitylation of its nuclear localization signal (NLS). |
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| Additional Comments | Cells with reduced levels of BAP1 exhibit chromosomal abnormalities and decreased DNA repair by homologous recombination, indicating that BAP1 dosage is critical. Cells with extensive DNA damage should die and not grow into malignancies. |
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| Bibliography | Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, Pellegrini L, Signorato V, Olivetto F, Pastorino S, Nasu M, Napolitano A, Gaudino G, Morris P, Sakamoto G, Ferris LK, Danese A, Raimondi A, Tacchetti C, Kuchay S, Pass HI, Affar EB, Yang H, Pinton P, Carbone M. BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature. 2017 Jun 22;546(7659):549-553. doi: 10.1038/nature22798. Epub 2017 Jun 14. PMID: 28614305; PMCID: PMC5581194. |
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