Primary Information |
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| BoMiProt ID | Bomi10216 |
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| Protein Name | Ubiquitin carboxyl-terminal hydrolase 20/Deubiquitinating enzyme 20/Ubiquitin thioesterase 20/Ubiquitin-specific-processing protease 20 |
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| Organism | Bos taurus |
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| Uniprot ID | A7Z056 |
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| Milk Fraction | Whey |
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| Ref Sequence ID | NP_001098803.1 |
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| Aminoacid Length | 912 |
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| Molecular Weight | 101815 |
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| FASTA Sequence |
Download |
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| Gene Name | USP20 |
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| Gene ID | 505839 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | Deubiquitinating enzyme.can deubiquitinate and stabilize the tumour suppressor protein p53 by reversing its nuclear export and degradation via Mdm2. |
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| Biochemical Properties | contains catalytic UCH domain which possess phosphorylated Ser76 site.Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin . |
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| PTMs | Phosphorylation and Ubl conjugation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A7Z056|UBP20_BOVIN Ubiquitin carboxyl-terminal hydrolase 20 OS=Bos taurus OX=9913 GN=USP20 PE=2 SV=1
MGDSRDLCPHLDSIGEVTKEDLLLKSKSTCQSCGVSGPNLWACLQVSCSYVGCGESFADH
STLHAQAKKHNLTVNLTTFRVWCYACEKEVFLEPRLAAHPPGPAPKFSEQDS*112PPPSHPLK
AVPIAVADEGES*132ES*134EDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLV
RTDKKPALCKSYQKLVSEVWHRKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLM
DQLHEELKEPVVATAAALT*259EARDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQG
RSGGGS*306QAEAELLMADEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALEQ
QPPETQPPS*369PRSTSPCRT*378PEPDNEAHMRSSRPCSPVHHHEGHAKLASS*408PHRAS*413PVRMGPAYVLKKAQVPGSRRRKEQSYRSVISDIFDGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGK
EDLAKLHSAIYQNVPAKPGACGDSYVAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDC
LAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKISSH
VSFPLEGLDLRPFLAKECTSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQ
YVTEVHETVVQNAEAYVLFYRKSSEEAVRERQQVVSLAAMREPSLLRFYVSREWLNKFNT
FAEPGPITNHTFLCSHGGIPPNKYHYIDDLVVILPQNVWEHLYSRFGGGPAVNHLYVCSI
CQVEIEALAKRRRVEIDTFIKLNKAFQAEESPSVIYCISMQWFREWEAFVKGKDNEPPGP
IDNSRIAQVKGSGHIQLKPGADYGQISEETWVYLNNLYGGGPEIAIRQSVAQLPDPESLH
GEQKIEAETRAL
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| Predicted Disorder Regions | 99-135, 259-434 |
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| DisProt Annotation | |
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| TM Helix Prediction | No TM helices |
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| Significance of PTMs | phosphorylation of USP10 at residues Thr42 and Ser337 by ATM pathway,promotesthe stability of USP10 and facilitate its translocation from the cytoplasm to the nucleus.Phosphorylated USP10 can inhibit the growth of tumour cells without inducing mutations in p53, which implies that phosphorylation of USP10 has a potential therapeutic effect against tumours.The deubiquitinase activity of USP10 is significantly increased when USP10 is phosphorylated at the Ser76 residue by AMPK under energy stress. |
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| Bibliography | Wang Y, Wang F. Post-Translational Modifications of Deubiquitinating Enzymes: Expanding the Ubiquitin Code. Front Pharmacol. 2021 Jun 10;12:685011. doi: 10.3389/fphar.2021.685011. PMID: 34177595; PMCID: PMC8224227. |
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