Primary Information
BoMiProt ID	Bomi10020
Protein Name	Transmembrane protein 65
Organism	Bos taurus
Uniprot ID	Q0VCH8
Milk Fraction	Whey
Ref Sequence ID	NP_001069786.1
Aminoacid Length	236
Molecular Weight	25041
FASTA Sequence	Download
Gene Name	TMEM65
Gene ID	614243
Protein Existence Status	reviewed
Secondary Information
Protein Function	TMEM65 is a mitochondrial inner-membrane protein.role in cardiac development and function.Tmem65 interacted with Cx43 (GJA1), a ventricular gap junction protein involved in rapid propagation of action potentials through myocardium.
Biochemical Properties	glycine zipper motif (GxxxGxxxG) within TMEM65 transmembrane domain 3.N- and C-terminal fragments of Tmem65 revealed that the N terminus was intracellular and the C terminus was extracellular.The N-terminal region (1-20) of this protein is sufficient for mitochondrial targeting and that this mitochondrial targeting signal (MTS) is cleaved between the amino acid positions 35 and 64, which contain a putative recognition site of matrix processing protease (MPP) suggesting that TMEM65 is imported into the mitochondria, integrated into mitochondrial inner-membrane, and processed into its mature form by an MPP.
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	na
Predicted Disorder Regions	3 disordered segments; (2-24), (45-60), (230-236)
DisProt Annotation
TM Helix Prediction	3TMHs; (112-134), (141-159), (207-225)
Significance of PTMs	post-translationally processed into a mature form.
Additional Comments	Knockdown of Tmem65 also reduced conductance velocity and synchronicity between Tmem65-knockdown cells.Knockdown of Tmem65 in neonatal mouse cardiomyocytes reduced localization of Cx43 to gap junctions.
Bibliography	Nishimura N, Gotoh T, Oike Y, Yano M. TMEM65 is a mitochondrial inner-membrane protein. PeerJ. 2014 Apr 10;2:e349. doi: 10.7717/peerj.349. PMID: 24765583; PMCID: PMC3994636.