Search by BoMiProt ID - Bomi9769


Primary Information

BoMiProt ID Bomi9769
Protein Name Terminal nucleotidyltransferase 5B
Organism Bos taurus
Uniprot IdQ29RH2
Milk FractionWhey
Ref Sequence Id NP_001039415.1
Aminoacid Length 430
Molecular Weight 47097
Fasta Sequence https://www.uniprot.org/uniprot/Q29RH2.fasta
Gene Name TENT5B/FAM46B
Gene Id 506745
Protein Existence Status reviewed

Secondary Information

Protein Function These are newly identified metazoan-specific poly(A) polymerases (PAPs),selectively add a string of nontemplated AMPs to the 3′-end of target mRNAs by consuming ATP molecules.
Biochemical Properties belong to nucleotidyltransferase (NTase) superfamily, a prominently larger N-terminal catalytic domain (NCD)as compared to known eukaryotic PAPs, and a C-terminal helical domain(HD).Asp91, Asp93 and Glu167 are present at the putative catalytic site.The NCD is built of a central eight-stranded β-sheet (β1−β8) surrounded by seven α-helices (α1−α7) involving a typical NTase core.The HD is composed of 5 α-helices which are associated with hydrophobic interactions.
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
na
Predicted Disorder Regions (1-47)
DisProt Annotation
TM Helix Prediction no TM helices
Additional Comments Knock-down of FAM46B induces apoptosis and restricts protein synthesis. FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin.
Bibliography 1.Hu JL, Liang H, Zhang H, Yang MZ, Sun W, Zhang P, Luo L, Feng JX, Bai H, Liu F, Zhang T, Yang JY, Gao Q, Long Y, Ma XY, Chen Y, Zhong Q, Yu B, Liao S, Wang Y, Zhao Y, Zeng MS, Cao N, Wang J, Chen W, Yang HT, Gao S. FAM46B is a prokaryotic-like cytoplasmic poly(A) polymerase essential in human embryonic stem cells. Nucleic Acids Res. 2020 Mar 18;48(5):2733-2748. doi: 10.1093/nar/gkaa049. PMID: 32009146; PMCID: PMC7049688. 2.Liang T, Ye X, Liu Y, Qiu X, Li Z, Tian B, Yan D. FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin. Exp Mol Med. 2018 Dec 10;50(12):1-12. doi: 10.1038/s12276-018-0184-0. PMID: 30532005; PMCID: PMC6288130.