Search by BoMiProt ID - Bomi94


Primary Information

BoMiProt ID Bomi94
Protein Name Thrombomodulin
Organism Bos taurus
Uniprot IDF1N6M2
Milk FractionWhey
Ref Sequence ID NP_001159994.1
Aminoacid Length 578
Molecular Weight 60730
FASTA Sequence Download
Gene Name THBD
Gene ID 281529
Protein Existence Status Unreviewed: Protein predicted

Secondary Information

Presence in other biological fluids/tissue/cells blood, lymph, urine, spinal fluid
Protein Function Converts thrombin to an anticoagulant; high affinity thrombin receptor; natural anticoagulant; molecular marker reflecting injury of endothelial cells; predictive marker of hypertensive complications in pregnancy
Biochemical Properties transmembrane proteoglycan receptor of thrombinl; cofactor of thrombin-catalyzed activation of protein C, and inhibits the procoagulant functions of thrombin; protein is very stable and compact because of several disulphur bridges; The long extracellular domain at the NH2-terminal part includes: a lectin-like domain necessary for TM endocytosis, a domain with 6 EGF-like repeats, among which the last three are necessary for protein C activation by thrombin, and a serine-threonine rich domain, putative sites of glycosylation responsible for the binding of a trisulphated chondroitin-sulphate, of importance in Plasmodium falciparum malaria; contains a hydrophobic transmembrane domain of 23 amino acids and a short intracytoplasmic tail probably associated with endocytosis and degradation.
PTMs As studied in modified human urinary thrombomodulin- N-linked oligosaccharides were assigned to Asn29, Asn98, Asn364, Asn391; those structures were estimated biantennary, 2-6 branched triantennary and 2-4 branched triantennary complex type oligosaccharides that were linked by fucose; attachment site of the GAG chain was assigned to Ser472; GAG chain contained chondroitin-4-sulfate and dermatan sulfate, which were repeated approximately 30 times
Bibliography 1. Esmon, C. T. (1995) Thrombomodulin as a model of molecular mechanisms that modulate protease specificity and function at the vessel surface. FASEB J. 9, 946–955.
2. Bourin, M. C., Lundgren-Akerlund, E., and Lindahl, U. (1990) Isolation and characterization of the glycosaminoglycan component of rabbit thrombomodulin proteoglycan. J. Biol. Chem. 265, 15424–15431.
3. Conway, E. M., Pollefeyt, S., Collen, D., and Steiner-Mosonyi, M. (1997) The amino terminal lectin-like domain of thrombomodulin is required for constitutive endocytosis. Blood 89, 652–661.
4. Gysin, J., Pouvelle, B., Le Tonquèze, M., Edelman, L., and Boffa, M. C. (1997) Chondroitin sulfate of thrombomodulin is an adhesion receptor for Plasmodium falciparum-infected erythrocytes. Mol. Biochem. Parasitol. 88, 267–271.
5. Boffa, M. C., Jackman, R. W., Peyri, N., Boffa, J. F., and George, B. (1991) Thrombomodulin in the central nervous system. Nouv. Rev. Fr. Hematol. 33, 423–429.
6. Boffa, M. C., Burke, B., and Haudenschild, C. C. (1987) Preservation of thrombomodulin antigen on vascular and extravascular surfaces. J. Histochem. Cytochem. 35, 1267–1276.
7. Pruna, A., Peyri, N., Berard, M., and Boffa, M. C. (1997) Thrombomodulin is synthesized by human mesangial cells. Kidney Int. 51, 687–693.