Primary Information |
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| BoMiProt ID | Bomi9135 |
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| Protein Name | Sharpin/Shank-associated RH domain-interacting protein |
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| Organism | Bos taurus |
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| Uniprot Id | E1BDF2 |
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| Milk Fraction | whey |
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| Ref Sequence Id | NP_001103236.1 |
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| Aminoacid Length | 409 |
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| Molecular Weight | 43394 |
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| Fasta Sequence | https://www.uniprot.org/uniprot/E1BDF2.fasta |
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| Gene Name | SHARPIN |
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| Gene Id | 512499 |
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| Protein Existence Status | Reviewed |
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Secondary Information |
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| Protein Function | It is a component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation |
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| Biochemical Properties | Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner |
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| PTMs | Phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|E1BDF2|SHRPN_BOVIN Sharpin OS=Bos taurus OX=9913 GN=SHARPIN PE=3 SV=1
MAPPAGGTAAGSDPGSAAVLLAVHVAVRPLGAGLDVAAQPRRLQLSADPERPGRFRLEML
GAGPGAVILEWPLESVSYTVRGPCQHELQPPPGGPGTLSLHFANPQEAQRWAALVRDATV
EGQNGSDSLPPALGPETRPVSPPSPLEVPTPKAPKPKVDLPWS*163PGDLMEKEELAGRLTRA
VEGGDEKGAAQAAAILAQRHVALRVQLQEAYFPPGPIRLQVTVEDAASSAHVSLQVHPHC
TIRALQEQVFSEFGFPPAVQRWVIGRCLCVPEHSLAFYGVQRDGDPAFLYLLSAPREAPG
RS*302PQRPQKVDGELGRLFPQSLGLPPTPQPTSSSLPSPLQPGWPCPSCTFINAPSRPGCEM
CSTQRPCAWDPLPTASIQQLPKVTRREDGPSLPGPRSLDPLLNLSGNLC |
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| Predicted Disorder Regions | 4-14, 38-40, 87-132, 142-182, 298-306, 321-339, 371-398 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Significance of PTMs | Serine 165 is the major phospho-acceptor site within SHARPIN.SHARPIN phosphorylation contributes to the optimal activation of NF-kB. |
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| Bibliography | 1.Thys A, Trillet K, Rosińska S, Gayraud A, Douanne T, Danger Y, Renaud CCN, Antigny L, Lavigne R, Pineau C, Com E, Vérité F, Gavard J, Bidère N. Serine 165 phosphorylation of SHARPIN regulates the activation of NF-κB. iScience. 2020 Dec 13;24(1):101939. doi: 10.1016/j.isci.2020.101939. PMID: 33392484; PMCID: PMC7773595. 2.Wang, Z., Potter, C. S., Sundberg, J. P., & Hogenesch, H. (2012). SHARPIN is a key regulator of immune and inflammatory responses. Journal of cellular and molecular medicine, 16(10), 2271–2279. https://doi.org/10.1111/j.1582-4934.2012.01574.x |
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