Search by BoMiProt ID - Bomi9057


Primary Information

BoMiProt ID Bomi9057
Protein Name Serine/threonine-protein kinase VRK1/Vaccinia-related kinase 1
Organism Bos taurus
Uniprot IdQ32PI1
Milk FractionWhey
Ref Sequence Id NP_001033313.1
Aminoacid Length 396
Molecular Weight 45358
Fasta Sequence https://www.uniprot.org/uniprot/Q32PI1.fasta
Gene Name VRK1
Gene Id 618880
Protein Existence Status reviewed

Secondary Information

Protein Function VRK1 regulates entry in cell cycle, chromatin condensation in G2/M, Golgi fragmentation, Cajal body dynamics and nuclear envelope assembly in mitosis. This kinase also controls the initial chromatin relaxation associated with histone acetylation, and the non-homologous-end joining (NHEJ) DNA repair pathway.
Biochemical Properties VRK1 has a strong autophosphorylating activity in several Ser and Thr residues.VRK-1 phosphorylates acidic proteins, such as phosvitin and casein, and basic proteins such as histone 2b and myelin basic protein.
PTMs Isopeptide bond, Phosphorylation at Ser, Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions 1-20, 331-336, 344-396
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes
Bibliography 1.Lopez-Borges S, Lazo PA. The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene. 2000 Jul 27;19(32):3656-64. doi: 10.1038/sj.onc.1203709. PMID: 10951572. 2.Campillo-Marcos I, García-González R, Navarro-Carrasco E, Lazo PA. The human VRK1 chromatin kinase in cancer biology. Cancer Lett. 2021 Apr 10;503:117-128. doi: 10.1016/j.canlet.2020.12.032. Epub 2021 Jan 29. PMID: 33516791. 3.Molitor TP, Traktman P. Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes. Mol Biol Cell. 2014 Mar;25(6):891-903. doi: 10.1091/mbc.E13-10-0603. Epub 2014 Jan 15. PMID: 24430874; PMCID: PMC3952857.