Search by BoMiProt ID - Bomi9049


Primary Information

BoMiProt ID Bomi9049
Protein Name Serine/threonine-protein kinase PLK1/Polo-like kinase 1/PLK-1
Organism Bos taurus
Uniprot IdQ2TA25
Milk FractionWhey
Ref Sequence Id NP_001033262.1
Aminoacid Length 602
Molecular Weight 68000
Fasta Sequence https://www.uniprot.org/uniprot/Q2TA25.fasta
Gene Name PLK1
Gene Id 538238
Protein Existence Status reviewed

Secondary Information

Protein Function Serine/threonine-protein kinase. regulates the activity of the histone kinases Aurora B and Haspin to define centromere identity, of MPS1 to initiate spindle checkpoint signaling, and of BUB1 and its pseudokinase paralog BUBR1 to coordinate spindle checkpoint activation and inactivation. .regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.
Biochemical Properties PLK1 is composed of 3 main domains, the serine/threonine kinase domain (KD), the inter domain linker (IDL), and the polo-box domain (PBD).T210 is localized in the activation loop starting at amino acid D194 of the DFG motif (Asp194, Phe195, Gly196). W414 and L490 are involved in substrate recognition via the PBD. H538 and K540 are important for specific binding to phosphorylated substrates (phospho-pincer region).
PTMs Phosphorylation,Ubl conjugation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
>sp|Q2TA25|PLK1_BOVIN Serine/threonine-protein kinase PLK1 OS=Bos taurus OX=9913 GN=PLK1 PE=2 SV=2 MSAAAT*6AGKLGRAPADPGKAPGVAAPGASTAAPPAKEIPEVLVDPRSRRRYLRGRFLGKG GFAKCFEISDADTKEVFAGKIVPKSLLLKPHQKEKMSMEIS*101IHRSLAHQHVVGFHGFFED NDFVFVVLELCRRRS*135LLELHKRRKALTEPEARYYLRQIVLGCQYLHGNRVIHRDLKLGNL FLNEDLEVKIGDFGLATKVEYDGERKKT*208LCGT*212PNYIAPEVLSKKGHSFEVDVWSIGCIMY TLLVGKPPFETSCLKETYLRIKNNEYS*267IPKHINPVASSLIKKMLQPDPTARPTIHELLND EFFTSGYIPARLPITCLTIPPRFSIAPSSLDPS*333NRKPLTVLNKGMENPMPERPREKEEPV VREASEPVDCHLS*373DMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYG LGYQLCDNSVGVLFNDSTRLILYSDGDS*448LQYIERDGSESYLTVSSHPNSLIKKITLLKYF RNYMSEHLLKAGANIT*496PREGDELARLPYLRTWFRTRSAIILHLSNGCVQINFFQDHTKLI LCPLMAAVTYIDEKRDFRTYRLSLLEEYGCSKELASRLRYARAMVDKLLSSRSAANRLKA SS
Predicted Disorder Regions 1-37, 346-396, 592-602
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Activated by phosphorylation of Thr-208 by AURKA.During G2/M transition,FoxM1 activity is enhanced by PLK1 phosphorylation, resulting in a positive feedback loop of increased expression of both PLK1 and Aurora B.The transcription factor Forkhead Box M1 (FoxM1) is important in regulating mitotic entry and subsequent execution of the mitotic program.in various cell types, or expression of a non-phosphorylatable FoxM1 results in decreased transcription of Aurora B. T210 is phosphorylated during G2 and mitosis whereas S137 is modified only during mitosis to activate PLK1. R337 and L340 are the 2 crucial residues of the D-box motif, and are essential for targeting PLK1 for degradation during late mitosis. K492 is mono-ubiquitylated by CUL3/KLHL22, allowing removal of PLK1 from the kinetochores and the metaphase to anaphase transition.
Bibliography 1.Schmucker S, Sumara I. Molecular dynamics of PLK1 during mitosis. Mol Cell Oncol. 2014 Oct 29;1(2):e954507. doi: 10.1080/23723548.2014.954507. PMID: 27308323; PMCID: PMC4905186. 2.Combes G, Alharbi I, Braga LG, Elowe S. Playing polo during mitosis: PLK1 takes the lead. Oncogene. 2017 Aug 24;36(34):4819-4827. doi: 10.1038/onc.2017.113. Epub 2017 Apr 24. PMID: 28436952.