Primary Information
BoMiProt ID	Bomi8382
Protein Name	Protein phosphatase 1 regulatory subunit 15A/Growth arrest and DNA damage-inducible protein GADD34
Organism	Bos taurus
Uniprot ID	Q2KI51
Milk Fraction	Whey
Ref Sequence ID	NP_001039643.1
Aminoacid Length	670
Molecular Weight	73053
FASTA Sequence	Download
Gene Name	PPP1R15A/GADD34
Gene ID	514688
Protein Existence Status	reviewed
Secondary Information
Protein Function	The major role of PPP1R15A is in the regulation of lung mesenchymal cells and regulation of PPP1R15A may represent a novel therapeutic strategy in IPF. A potential protective role for PPP1R15A in both the initiation and maintenance of lung fibrosis.
Significance in milk	This gene was found to be downregulated in response to Methionine supply in periparturient Holstein cows.
PTMs	Phosphorylation at Tyr, Ubl conjugation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|Q2KI51|PR15A_BOVIN Protein phosphatase 1 regulatory subunit 15A OS=Bos taurus OX=9913 GN=PPP1R15A PE=2 SV=2 MAPGQMPHQPAPWRGTHPLFLLSPLMGLLSRAWSLLRAPGPPEPWLVEAVTEADQGGAGL EDEAKASLATYHALWGRHPQEETKDSGAAEEDREASPGACPNLEAKHSLPEAWGLSDDDD EKYGGEEATGVPREQKEFMDGQPAPLPLSLLIRSLPDLPGEEESKEEAVTGGGGNEVTAF SFPLSHWECCPGEEEEEEEENGEAVRVCRPVNGATEERTQTEAATKTSMSPSSVGSHLRA WECCSGKESEEEEKDKQAEKGDADPGPHFTSLAQRPSLRTWQHPSSAITEEEEDRDSEEM GASSSVPLTSAFLSDWVYQPEDTEEEDEEEEDCDSEATEDEGEAEVSSATPPPSAFLSAW VYRPGEDTEEEEDCDSEATEDEGEAEVSSATPPTSAFLSAWVY*403QPGDTEEEEDCDSEATE DEGEAEVSSATPPPSAFLSAWVY*443RPGEDTEEEDEYEDEDNESGAADLGPSPSLQTQSALL RDQIYQPGEKTDGGEAAEKWGEAESCPFRVAIY*513LPGEKPPPPWDPPRLPLRLQRRLKSAQ TPTRHQDLERLLKTRKVRFSEKVSIHPLVVWAGPAQAARRGPWEQFARDRSRFARRIAQV QEELGPYLTPAARARAWARLGNPPTSLATVPAPTQTSPMTPIQATPLSHALASPSPPCVS PSLDLSGRRG
Predicted Disorder Regions	19-29, 62-491, 571-585
DisProt Annotation
TM Helix Prediction	No TM helices
Significance of PTMs	Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
Additional Comments	Interestingly, the loss of PPP1R15A appeared to promote lung fibroblast senescence.
Bibliography	1.Monkley S, Overed-Sayer C, Parfrey H, Rassl D, Crowther D, Escudero-Ibarz L, Davis N, Carruthers A, Berks R, Coetzee M, Kolosionek E, Karlsson M, Griffin LR, Clausen M, Belfield G, Hogaboam CM, Murray LA. Sensitization of the UPR by loss of PPP1R15A promotes fibrosis and senescence in IPF. Sci Rep. 2021 Nov 3;11(1):21584. doi: 10.1038/s41598-021-00769-7. PMID: 34732748; PMCID: PMC8566588. 2.Ma YF, Batistel F, Xu TL, Han LQ, Bucktrout R, Liang Y, Coleman DN, Parys C, Loor JJ. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci. 2019 May;102(5):4264-4274. doi: 10.3168/jds.2018-15451. Epub 2019 Mar 14. PMID: 30879806.