Primary Information |
|---|
| BoMiProt ID | Bomi8001 |
|---|
| Protein Name | Phospholipase D2 |
|---|
| Organism | Bos taurus |
|---|
| Uniprot ID | Q0V8L6 |
|---|
| Milk Fraction | Whey,MFGM |
|---|
| Ref Sequence ID | NP_001069295.1 |
|---|
| Aminoacid Length | 933 |
|---|
| Molecular Weight | 105766 |
|---|
| FASTA Sequence |
Download |
|---|
| Gene Name | PLD2 |
|---|
| Gene ID | 522159 |
|---|
| Protein Existence Status | reviewed |
|---|
Secondary Information |
|---|
| Protein Function | PLD2 promotes PTPN14-mediated dephosphorylation of VE-cadherin and that redistribution of VE-cadherin at adherens junctions is essential for recovery of endothelial barrier function after an edemagenic insult. |
|---|
| Biochemical Properties | PLD generated PA mediates a variety of cellular functions, including growth/proliferation, vesicle trafficking, cytoskeletal rearrangement and activation of NADPH Oxidase (NOX), and most of its functions are attributed to the broad interactions with other proteins and lipids. |
|---|
| PTMs | Phosphorylation and Ubiquitinated at Lys-454,466 residue |
|---|
Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| NA |
|---|
| Predicted Disorder Regions | 1-36, 139-156 |
|---|
| DisProt Annotation | |
|---|
| TM Helix Prediction | No TM helices |
|---|
| Significance of PTMs | RNF145 triggers ubiquitination of SCAP, likely inhibiting SCAP-SREBP complex and transport it to the Golgi apparatus and the subsequent maturation of SREBF2/SREBP2. |
|---|
| Additional Comments | PLD2 protein co-expresses with chromogranin A in castrate-resistant prostate cancer tissue. PLD2 inhibition reduces prostate cancer cell viability, colony forming ability and directional cell movement. |
|---|
| Bibliography | 1.Fu P, Ramchandran R, Shaaya M, Huang L, Ebenezer DL, Jiang Y, Komarova Y, Vogel SM, Malik AB, Minshall RD, Du G, Tonks NK, Natarajan V. Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation. J Biol Chem. 2020 May 29;295(22):7669-7685. doi: 10.1074/jbc.RA119.011801. Epub 2020 Apr 23. PMID: 32327488; PMCID: PMC7261798. 2.Noble AR, Hogg K, Suman R, Berney DM, Bourgoin S, Maitland NJ, Rumsby MG. Phospholipase D2 in prostate cancer: protein expression changes with Gleason score. Br J Cancer. 2019 Dec;121(12):1016-1026. doi: 10.1038/s41416-019-0610-7. Epub 2019 Nov 1. PMID: 31673104; PMCID: PMC6964697. |
|---|
