Primary Information |
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| BoMiProt ID | Bomi7923 |
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| Protein Name | PHD finger protein 23/PDH-containing protein JUNE-1 |
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| Organism | Bos taurus |
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| Uniprot Id | A5D962 |
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| Milk Fraction | Whey |
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| Ref Sequence Id | NP_001019741.1 |
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| Aminoacid Length | 400 |
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| Molecular Weight | 43440 |
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| Fasta Sequence | https://www.uniprot.org/uniprot/A5D962.fasta |
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| Gene Name | PHF23 |
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| Gene Id | 539774 |
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| Protein Existence Status | reviewed |
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Secondary Information |
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| Protein Function | negative regulates autophagy and mitophagy.PHF23 interacts with LRSAM1, which is an E3 ligase key for ubiquitin-dependent autophagy against invading bacteria. |
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| Biochemical Properties | PHD finger of PHF23 is a functional domain needed for the interaction with LRSAM1.PHD domain of PHF23 can specially recognize histone H3 lysine 4 trimethylation (H3K4me3) which is suggested as a pivotal component in the regulation of gene expression and epigenetic state. Plant homeodomain (PHD)-like zinc finger domain at the C terminus, is characterized by a canonical Cys4-His-Cys3 (or C4HC3) motif that coordinates 2 zinc ions .It is predicted to contain a nuclear localization signal (residues 177 to 228). |
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| PTMs | Acetylation and phosphorylation |
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Site(s) of PTM(s)
N-glycosylation,
O-glycosylation,
Phosphorylation
| >sp|A5D962|PHF23_BOVIN PHD finger protein 23 OS=Bos taurus OX=9913 GN=PHF23 PE=2 SV=1
MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPAS
GSSSPLRGESAADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFPRLQ
APDS*124ATLLEKMKLKDSLFDIDGPKMAS*147PLS*150PTSLTHASRPPAALT*165PVPLSQGDLSQPPRKKDRKNRKLGPGGATGFGVLRRPRPAPGDGEKRSRIKKSKKRKLKKAERGDRLPPPGPPRA
PPSDTDSEEEEEEEEEEEEMAAMVGGEAPAPVLPTPEAPRPPATVHPEGAPPTDGESKEV
GSTETSQDGDAS*312S*313S*314EGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPMIECSLCGTWI
HLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKS*397GEP
|
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| Predicted Disorder Regions | 1-318, 386-400 |
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| DisProt Annotation | |
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| TM Helix Prediction | no TM helices |
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| Additional Comments | 1.Knockdown of PHF23 protects chondrocytes from IL-1β induced osteoarthritis by enhancing autophagy. |
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| Bibliography | 1.Maimaitijuma T, Yu JH, Ren YL, Yang X, Liu H, Meng ZC, Wang R, Cui YP, Wu H, Pan LP, Jiao Y, Chen YY, Cao YP. PHF23 negatively regulates the autophagy of chondrocytes in osteoarthritis. Life Sci. 2020 Jul 15;253:117750. doi: 10.1016/j.lfs.2020.117750. Epub 2020 May 5. PMID: 32380078. 2.Wang Z, Hu J, Li G, Qu L, He Q, Lou Y, Song Q, Ma D, Chen Y. PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy. 2014;10(12):2158-70. doi: 10.4161/auto.36439. PMID: 25484098; PMCID: PMC4502667. |
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