Primary Information
BoMiProt ID	Bomi7923
Protein Name	PHD finger protein 23/PDH-containing protein JUNE-1
Organism	Bos taurus
Uniprot ID	A5D962
Milk Fraction	Whey
Ref Sequence ID	NP_001019741.1
Aminoacid Length	400
Molecular Weight	43440
FASTA Sequence	Download
Gene Name	PHF23
Gene ID	539774
Protein Existence Status	reviewed
Secondary Information
Protein Function	negative regulates autophagy and mitophagy.PHF23 interacts with LRSAM1, which is an E3 ligase key for ubiquitin-dependent autophagy against invading bacteria.
Biochemical Properties	PHD finger of PHF23 is a functional domain needed for the interaction with LRSAM1.PHD domain of PHF23 can specially recognize histone H3 lysine 4 trimethylation (H3K4me3) which is suggested as a pivotal component in the regulation of gene expression and epigenetic state. Plant homeodomain (PHD)-like zinc finger domain at the C terminus, is characterized by a canonical Cys4-His-Cys3 (or C4HC3) motif that coordinates 2 zinc ions .It is predicted to contain a nuclear localization signal (residues 177 to 228).
PTMs	Acetylation and phosphorylation
Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation	>sp|A5D962|PHF23_BOVIN PHD finger protein 23 OS=Bos taurus OX=9913 GN=PHF23 PE=2 SV=1 MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPAS GSSSPLRGESAADSDGWDSAPSDLRTIQTFVKKAKSSKRRAAQAGPTQPGPPRSTFPRLQ APDS*124ATLLEKMKLKDSLFDIDGPKMAS*147PLS*150PTSLTHASRPPAALT*165PVPLSQGDLSQPPRKKDRKNRKLGPGGATGFGVLRRPRPAPGDGEKRSRIKKSKKRKLKKAERGDRLPPPGPPRA PPSDTDSEEEEEEEEEEEEMAAMVGGEAPAPVLPTPEAPRPPATVHPEGAPPTDGESKEV GSTETSQDGDAS*312S*313S*314EGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRPMIECSLCGTWI HLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGPPKS*397GEP
Predicted Disorder Regions	1-318, 386-400
DisProt Annotation
TM Helix Prediction	no TM helices
Additional Comments	1.Knockdown of PHF23 protects chondrocytes from IL-1β induced osteoarthritis by enhancing autophagy.
Bibliography	1.Maimaitijuma T, Yu JH, Ren YL, Yang X, Liu H, Meng ZC, Wang R, Cui YP, Wu H, Pan LP, Jiao Y, Chen YY, Cao YP. PHF23 negatively regulates the autophagy of chondrocytes in osteoarthritis. Life Sci. 2020 Jul 15;253:117750. doi: 10.1016/j.lfs.2020.117750. Epub 2020 May 5. PMID: 32380078. 2.Wang Z, Hu J, Li G, Qu L, He Q, Lou Y, Song Q, Ma D, Chen Y. PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy. 2014;10(12):2158-70. doi: 10.4161/auto.36439. PMID: 25484098; PMCID: PMC4502667.