Search by BoMiProt ID - Bomi7396


Primary Information

BoMiProt ID Bomi7396
Protein Name NEDD8/Neddylin/Ubiquitin-like protein Nedd8
Organism Bos taurus
Uniprot IdP61282
Milk FractionExosomes
Ref Sequence Id NP_777189.1
Aminoacid Length 81
Molecular Weight 9072
Fasta Sequence https://www.uniprot.org/uniprot/P61282.fasta
Gene Name NEDD8
Gene Id 286796
Protein Existence Status Reviewed

Secondary Information

Protein Function The ubiquitin-related protein NEDD8 is conjugated and deconjugated to and from proteins in processes related to ubiquitin conjugation and deconjugation. 
Biochemical Properties NEDD8 is nearly encircled by interactions, binding its linked CUL1 WHB domain in an “activation module”, and positioning a “catalytic module” relative to the substrate scaffolding module to juxtapose the active site and substrate.
PTMs N6-Acetylation at Lys, Isopeptide bond formation,Cleavage by UCHL3 or SENP8
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Significance of PTMs Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
Additional Comments NEDD8 Overexpression Results in Neddylation of Ubiquitin Substrates by the Ubiquitin Pathway
Bibliography 1.Schwechheimer C. NEDD8-its role in the regulation of Cullin-RING ligases. Curr Opin Plant Biol. 2018 Oct;45(Pt A):112-119. doi: 10.1016/j.pbi.2018.05.017. Epub 2018 Jun 15. PMID: 29909289. 2.Baek K, Krist DT, Prabu JR, Hill S, Klügel M, Neumaier LM, von Gronau S, Kleiger G, Schulman BA. NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly. Nature. 2020 Feb;578(7795):461-466. doi: 10.1038/s41586-020-2000-y. Epub 2020 Feb 12. PMID: 32051583; PMCID: PMC7050210. 3.Roland Hjerpe; Yann Thomas; Thimo Kurz (2012). NEDD8 Overexpression Results in Neddylation of Ubiquitin Substrates by the Ubiquitin Pathway. , 421(1), –. doi:10.1016/j.jmb.2012.05.013.