Search by BoMiProt ID - Bomi7359


Primary Information

BoMiProt ID Bomi7359
Protein Name NAD-capped RNA hydrolase NUDT12
Organism Bos taurus
Uniprot IdQ29RH3
Milk FractionWhey
Ref Sequence Id NP_001040073.1
Aminoacid Length 444
Molecular Weight 50119
Fasta Sequence https://www.uniprot.org/uniprot/Q29RH3.fasta
Gene Name NUDT12
Gene Id 617720
Protein Existence Status reviewed

Secondary Information

Protein Function Nudt12 in complex with the deNADding product AMP and three Mg2+ ions at 1.6 Å resolution provides insights into the molecular basis of the deNADding activity in the NAD pyrophosphate.
Biochemical Properties NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets. We identify an ∼600-kDa dodecamer complex between bleomycin hydrolase (BLMH) and NUDT12, with BLMH being required for localization of NUDT12 to a few discrete cytoplasmic granules that are distinct from P-bodies. 
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions NA
DisProt Annotation
TM Helix Prediction No TM helices
Additional Comments , loss of Nudt12 results in a significant upregulation of circadian clock transcripts in mouse liver.
Bibliography 1.Grudzien-Nogalska E, Wu Y, Jiao X, Cui H, Mateyak MK, Hart RP, Tong L, Kiledjian M. Structural and mechanistic basis of mammalian Nudt12 RNA deNADding. Nat Chem Biol. 2019 Jun;15(6):575-582. doi: 10.1038/s41589-019-0293-7. Epub 2019 May 17. PMID: 31101919; PMCID: PMC6527130.