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Primary Information | |
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| BoMiProt ID | Bomi726 |
| Protein Name | DnaJ homolog subfamily C member 3 |
| Organism | Bos taurus |
| Uniprot ID | Q27968 |
| Milk Fraction | Whey |
| Ref Sequence ID | NP_777181.1 |
| Aminoacid Length | 504 |
| Molecular Weight | 57704 |
| FASTA Sequence | Download |
| Gene Name | DNAJC3 |
| Gene ID | 286770 |
| Protein Existence Status | Reviewed: Experimental evidence at protein level |
Secondary Information | |
| Protein Function | DnaJ proteins, also known as heat shock protein 40 (Hsp40 or Hsc40) act as cochaperones to the molecular chaperone DnaK (Hsp70), which is responsible for several cellular processes such as rescuing misfolded proteins, folding polypeptide chains, transport of polypeptides through membranes, assembly and disassembly of protein complexes, and control of regulatory proteins; primarily facilitates the hydrolysis of ATP from DnaK that is necessary for the chaperone activity of DnaK; DNAJA1 appears to be involved in importing proteins into the mitochondria |
| Significance in milk | heat stress proteins in mamary gland |
| Linking IDs | Bomi56 |
| Bibliography | 1. Li, L., Sun, Y., Wu, J., Li, X., Luo, M., & Wang, G. (2015). The global effect of heat on gene expression in cultured bovine mammary epithelial cells. Cell Stress & Chaperones, 20(2), 381–389. https://doi.org/10.1007/s12192-014-0559-7. 2. Stark, J. L., Mehla, K., Chaika, N., Acton, T. B., Xiao, R., Singh, P. K., … Powers, R. (2014). Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer. Biochemistry, 53(8), 1360–1372. https://doi.org/10.1021/bi401329a. |
| Protein Function | 58-kilodalton inhibitor of protein kinase (P58(IPK)) plays an important role in preventing endoplasmic reticulum (ER) stress. |
| PTMs | Phosphorylation at Serine,Disulfide bond formation |
| Site(s) of PTM(s) N-glycosylation, O-glycosylation, Phosphorylation | |
| Predicted Disorder Regions | (451-481) |
| DisProt Annotation | |
| TM Helix Prediction | No TM helices |
| Additional Comments | Overexpression of P58(IPK) downregulated the expression of CHOP, TNF-α, and VEGF under high glucose conditions, whereas introduction of P58(IPK)RNAi enhanced the expression of CHOP, TNF-α, and VEGF. |
| Bibliography | 1.Yang H, Liu R, Cui Z, Chen ZQ, Yan S, Pei H, Li B. Functional characterization of 58-kilodalton inhibitor of protein kinase in protecting against diabetic retinopathy via the endoplasmic reticulum stress pathway. Mol Vis. 2011 Jan 10;17:78-84. PMID: 21245960; PMCID: PMC3021576. |