Search by BoMiProt ID - Bomi7129


Primary Information

BoMiProt ID Bomi7129
Protein Name Migration and invasion enhancer 1
Organism Bos taurus
Uniprot IdQ148C8
Milk FractionWhey
Ref Sequence Id NP_001068689.1
Aminoacid Length 115
Molecular Weight 12342
Fasta Sequence https://www.uniprot.org/uniprot/Q148C8.fasta
Gene Name MIEN1
Gene Id 505710
Protein Existence Status reviewed

Secondary Information

Protein Function involved in cytoskeletal focal adhesion dynamics.Increases cell migration by inducing filopodia formation.Also considered an oncogenic protein, because MIEN1 overexpression functionally enhances migration and invasion of tumor cells via modulating the activity of AKT.
Biochemical Properties MIEN1 protein contains a consensus sequence for prenylation comprising the last 4 amino acids, CVIL, at the C terminus, a CAAX motif predicted to be post-translationally modified by protein geranylgeranyltransferase-1.It contains a central α/β core domain consisting of a mixed parallel/anti-parallel four-stranded β-sheet with two distorted α-helices.
PTMs Acetylation, Disulfide bond formation, Lipidation, Prenylation
Site(s) of PTM(s)

N-glycosylation, O-glycosylation,
Phosphorylation
NA
Predicted Disorder Regions 1-23, 99-103, 108-111
DisProt Annotation
TM Helix Prediction 6TMHs; (52-74), (85-107), (130-148), (161-183), (217-239), (252-270)
Significance of PTMs contains a consensus sequence for prenylation comprising the last 4 amino acids.Geranylation of MIEN1 at the CAAX motif facilitated the association of the protein to the inner leaflet of plasma membrane, enhancing the migratory phenotype of cells by inducing increased filopodia formation and potentiating directional migration.-CAAX- motif in the C terminal is predicted to be post-translationally modified by protein geranylgeranyltransferase-1.
Bibliography 1.Hsu CH, Shen TL, Chang CF, Chang YY, Huang LY. Solution structure of the oncogenic MIEN1 protein reveals a thioredoxin-like fold with a redox-active motif. PLoS One. 2012;7(12):e52292. doi: 10.1371/journal.pone.0052292. Epub 2012 Dec 20. PMID: 23284973; PMCID: PMC3527542.